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Whole Transcriptome analysis of the coral acropora millepora reveals complex responses to ocean acidification during the initiation of calcification

ABSTRACT: We used the Illumina RNAseq approach to study the effects of acute exposure to elevated CO2 on gene expression in primary polyps of Acropora millepora

ORGANISM(S): Acropora millepora

PROVIDER: GSE33016 | GEO | 2012/04/10

SECONDARY ACCESSION(S): PRJNA149513

REPOSITORIES: GEO

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Whole Transcriptome analysis of the coral acropora millepora reveals complex responses to ocean acidification during the initiation of calcification

Project description:We used the Illumina RNAseq approach to study the effects of acute exposure to elevated CO2 on gene expression in primary polyps of Acropora millepora Examination of transcriptome in Acropora millepora primary polyps at 380, 750 and 1000 ppm CO2 after 3 days exposure

2012-04-10 | E-GEOD-33016 | biostudies-arrayexpress

Acropora millepora adult samples: Control vs medium and high ocean acidification treatment

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Rapid acclimation of juvenile corals to CO2-mediated acidification by up-regulation of HSP and Bcl-2 genes

Project description:We compared the effect of acute (Moya et al 2012) and chronic exposure to elevated pCO2 on gene expression in primary polyps of Acropora millepora Examination of transcriptome in Acropora millepora primary polyps at 380 and 750 ppm CO2 (air) after 9 days exposure

2015-05-01 | E-GEOD-61114 | biostudies-arrayexpress

Acropora millepora adult samples: Control vs medium and high ocean acidification treatment

Project description:In this study we investigated how changes in pH and ocean chemistry consistent with the scenarios of the Intergovernmental Panel on Climate Change (IPCC) drive major changes in gene expression, respiration, photosynthesis and symbiosis of the coral, Acropora millepora, long before they affect biomineralization. Changes in gene expression were consistent with metabolic suppression, an increase in oxidative stress, apoptosis and symbiont loss. Other expression patterns demonstrated up-regulation of membrane transporters, as well as the regulation of genes involved in membrane cytoskeletal interactions and cytoskeletal remodeling. These widespread changes in gene expression emphasize the need to expand future studies of ocean acidification to include a wider spectrum of cellular processes, many of which may occur well before impacts on calcification. We applied a reference microarray design for the experiment outlined in the study, which was a three condition experiment of ocean acidification: control pH 8.0-8.2, medium pH 7.8-7.9 and high pH 7.6-7.7, and across three time points: time zero, day 1 and day 28. Samples from time zero and control treatments were used to generate the reference sample for the microarray hybridization experiments. A total of 27 microarrays were used in the entire experiment, 3 biological replicates per treatment and timepoint. Reference samples in each array was labeled with Cy3, and the actual experimental samples with Cy5.

2012-07-29 | E-GEOD-28697 | biostudies-arrayexpress

Molecular response of the coral Acropora millepora to three settlement and/or metamorphosis induced stimuli

Project description:Two known settlement/metamorphosis inducing stimuli (crustose coralline algae, and ethanolic extract of crustose coralline algae) and one stimulus which just induces metamorphosis (LWamide) were used to stimulate competent planula larvae of the coral Acropora millepora. Samples were taken 0.5h, 4h and 12h post induction isolate the genes controlling settlement and metamorphosis in this coral.

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Project description:Acropora millepora transcriptome project

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Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals

Project description:The metabolic bases of the interaction between the coral Acropora millepora and its dinoflagellate symbiont were investigated by comparing gene expression levels under light and dark conditions at the whole transcriptome level. Among the differentially expressed genes identified, a suite of genes involved in cholesterol transport was found to be up-regulated under light conditions, confirming the significance of this compound in the coral symbiosis. Although ion transporters likely to have roles in calcification were not differentially expressed in this study, expression levels of many genes associated with skeletal organic matrix composition and organization were higher in light conditions. This implies that the rate of organic matrix synthesis is one factor limiting calcification at night. Thus, LEC during the day is likely to be a consequence of increases in both matrix synthesis and the supply of precursor molecules as a result of photosynthetic activity. Branch tips from three adult colonies of Acropora millepora were sampled at midday and midnight

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