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Jejunal and pancreatic transcriptomic adaptations underpin enhanced performance in broilers fed sugarcane bagasse-supplemented diets

ABSTRACT: This study investigated the transcriptomic changes in the jejunum and pancreas of broiler chickens fed a diet supplemented with sugarcane bagasse (SB) to elucidate the physiological response of broilers to insoluble dietary fiber. Differentially expressed genes (DEGs) were identified (significant threshold at absolute log2 (fold change) ≥ 1 and P < 0.05) and functionally annotated using gene ontology (GO; gene counts ≥ 2 and P < 0.05 as significant) analysis. Compared with the control broilers, SB-fed broilers presented significantly (P < 0.05) greater weight gain and improved feed conversion ratio at the end of the grower phase. A total of 41 (18 upregulated and 23 downregulated) jejunal and 125 (36 upregulated and 89 downregulated) pancreatic DEGs were identified from the differential expression analysis. The SB-fed broilers showed coordinated upregulation of DEGs involved in nutrient transport (TRPM3, SLC16A4, FFAR4, and RBP4A), epithelial integrity (WNT9A, GAL3ST2, TFF3, and AGR2), immune activation (DUOX2 and MHCY6), and growth regulation (POU1F1). Gene ontology enrichment further revealed significant activation of biological processes, including maintenance of the gastrointestinal epithelium, gastric acid secretion, sodium ion transport, and response to oxidative stress, in the pancreatic tissue. These findings reveal that SB supplementation triggers beneficial, tissue-specific transcriptomic adaptations that support nutrient uptake, epithelial repair, immune response, and oxidative balance, thereby enhancing growth. This study offers new insights into how broilers respond to dietary fiber supplementation at the transcriptomic level, supporting the strategic use of SB in sustainable poultry production.

ORGANISM(S): Gallus gallus

PROVIDER: GSE330384 | GEO | 2026/05/13

REPOSITORIES: GEO

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Project description:The aim of this study was to investigate whether long term intake of pea fiber would improve colonic barrier, bacterial profile and alter colonic gene expression using DNA microarray. Fifty weaned pigs were randomly allocated into 2 groups receiving control and fibrous diet with inclusion of pea fiber from weaning age until d 160. The two diets had similar nutrient levels. Pigs fed pea fiber diet (PF diet) had markedly decreased overall average daily feed intake (ADFI) and Feed:Gain in growing and finishing period (P<0.05). In addition, long term intake of PF diet induced deeper crypt (+50 %, P<0.05), increased protein expression of colonic mucin and sIgA (+13～16 %, P<0.05). Resulting from the increased lactobacillus content (P<0.05), moreover, pigs fed PF diet had significantly higher concentration of colonic total short chain fatty acid (SCFA) and acetic acid. DNA microarray results indicated that feeding PF diet induced alterations in the expression of colonic cancer, immune response and lipid metabolism-related genes, as well as genes involved in signal pathway such as intestinal immune network for IgA production, PPAR signaling pathway and nutrient metabolism-related pathways. Collectively, our results suggested that long term intake of PF diet would improve colonic health via altering colonic bacteria profile, colonic barriers, immune and metabolism related protein or gene expressions. A total of 50 weaned pigs (Duroc×Landrace×Yorkshire, initial body weight: 7.2±0.5 kg) were randomly allocated to 2 groups with 5 pens each group and 5 pig each pen. Pigs were fed control (Control) and fibrous diets (10～20 % inclusion of pea fiber, PF) from weaning at 28 day to 160 day-old-age, which is subjected to phase feeding by weaning diet (weaning to d 30 post-weaning), growing diet (d 30～90 postweaning) and finishing diet (d 90～160 postweaning) according to their physiological stage. At d 160 postweaning, four pigs each group were selected to be slaughtered for collection of colonic tissues and DNA microarray was applied to the colonic tissues for analysis of gene expression.

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Jejunal and pancreatic transcriptomic adaptations underpin enhanced performance in broilers fed sugarcane bagasse-supplemented diets

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