Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in response to the expression of the bacterial type 3 effector DspA/E

ABSTRACT: au10-11_dspa - Induction of DspA/E expression in a transgenic A. thaliana line - Does DspA/E repress defenses at the transcriptionnal level? What defenses are triggered directly in response to the perception of DspA/E? - Transgenic lines expressing a GFP::DspA/E fusion or GFP alone were treated with estradiol or with DMSO. Sample were collected 6 hours following treatment.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE33046 | GEO | 2011/11/01

SECONDARY ACCESSION(S): PRJNA149471

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression in response to the expression of the bacterial type 3 effector DspA/E

Project description:au10-11_dspa - Induction of DspA/E expression in a transgenic A. thaliana line - Does DspA/E repress defenses at the transcriptionnal level? What defenses are triggered directly in response to the perception of DspA/E? - Transgenic lines expressing a GFP::DspA/E fusion or GFP alone were treated with estradiol or with DMSO. Sample were collected 6 hours following treatment. 8 dye-swap - gene knock out,genotype comparaison,treated vs untreated comparison

2011-11-01 | E-GEOD-33046 | biostudies-arrayexpress

au10-01_overexpressionoxi1-Transcriptomic analysis of the overexpression of Oxi1

Project description:au10-01_overexpressionoxi1 - au10-01_overexpressionoxi1 - Analysis of the overexpression of the Oxi1 - Comparision between the overexpression of oxi1 and wt. 2 dye-swap - normal vs transgenic comparison

2012-05-31 | E-GEOD-35941 | biostudies-arrayexpress

defenses mechanism in response to cold stress-PI4kinases and Long-chain bases kinases in response to cold stress

Project description:au10-10_froid - defenses mechanism in response to cold stress - Role of PI4 kinase and Long-chain bases kinases in response to cold stress - Plantlets of wild type and mutant were grown vitro for 14 days in a growth chamber (80% humidity, 80 micro Enstein without photoperiod). The 15th day, plantlets were transferred at 4°C or 12°C during 4 hours (80 micro Enstein) for the cold stress.

2012-03-23 | GSE36624 | GEO

defenses mechanism in response to cold stress-PI4kinases and Long-chain bases kinases in response to cold stress

Project description:au10-10_froid - defenses mechanism in response to cold stress - Role of PI4 kinase and Long-chain bases kinases in response to cold stress - Plantlets of wild type and mutant were grown vitro for 14 days in a growth chamber (80% humidity, 80 micro Enstein without photoperiod). The 15th day, plantlets were transferred at 4Â°C or 12Â°C during 4 hours (80 micro Enstein) for the cold stress. 14 dye-swap - treated vs untreated comparison

2012-03-23 | E-GEOD-36624 | biostudies-arrayexpress

The PXC3 receptor-like kinase constitutively interacts with BAK1 and is involved in vascular development in Arabidopsis.

Project description:We designed an experiment to identify BAK1 interactors considering that it could be a coreceptor for the GOLVEN6 (GLV6) signaling peptide involved in lateral root organogenesis in Arabidopsis thaliana. A BAK1pro:BAK1-GFP/bak1-4 line crossed to an estradiol-inducible GLV6 overexpression line (BAK1pro:BAK1-GFP/bak1-4xiGLV6) was treated with mock or estradiol followed by affinity purification mass spectrometry (AP-MS) experiments using anti-GFP beads in microsomal-enriched fractions. Statistical analysis of estradiol-treated vs. mock seedlings did not reveal significant differences. Nevertheless, we remarked that regardless of the treatment, several LRR-RLKs co-eluted with BAK1-GFP, comprising known, as well as, unknown interactors. We confirmed interaction of BAK1 with these LRR-RLKs by BIFC and identified the PXC3 receptor as a BAK1 constitutive interactor, which was further verified by co-IP.

2022-02-17 | PXD026487 | Pride

Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plants (gemsupv2ts-7)

Project description:au10-09_gemsupv2ts-7 - gemsupv2ts-7 - Determine the level of expression in transgenic plants expressing geminiviral gene silencing suppressors. - One transgenic line of Arabidopsis overexpressing the geminiviral silencing suppressor V2TS was grown in adition to a control and their total RNA was extracted in order to study its genetic expression profile.

2011-08-01 | GSE30766 | GEO

The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector

Project description:Plant immunity is tightly controlled by a complex and dynamic regulatory network, which ensures optimal activation upon detection of potential pathogens. Accordingly, each component of this network is a potential target for manipulation by pathogens. Here, we report that RipAC, a type III-secreted effector from the bacterial pathogen Ralstonia solanacearum, targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern-triggered immunity (PTI). PUB4 plays a positive role in PTI by regulating the homeostasis of the central immune kinase BIK1. Before PAMP perception, PUB4 promotes the degradation of non-activated BIK1, while, after PAMP perception, PUB4 contributes to the accumulation of activated BIK1. RipAC leads to BIK1 degradation, which correlates with its PTI-inhibitory activity. RipAC causes a reduction in pathogen-associated molecular pattern (PAMP)-induced PUB4 accumulation and phosphorylation. Our results shed light on the role played by PUB4 in immune regulation, and illustrate an indirect targeting of the immune signalling hub BIK1 by a bacterial effector

2022-10-12 | PXD036739 | Pride

Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plants (gemsupv2ts-7)

2011-07-31 | E-GEOD-30766 | biostudies-arrayexpress

Transcriptomic changes in Arabidopsis thaliana upon the expression of mutated TFIIS

Project description:The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of transcriptomic changes upon TFIISmut expression was performed by RNA-seq to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by RNA-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction.

2020-03-25 | GSE129072 | GEO

Alteration in liver proteome caused by ethinyl estradiol in males Cyprinodon variegatus

Project description:Adult male marine fish were exposed to 100ng/L of ethinyl estradiol (EE2) for 7 days in 1.8L vessels, dimethyl sulfoxide (DMSO) was used as carrier never reaching concentrations > 0.001% v/v. The same volume of DMSO was added to the solvent control vessels. Culture conditions, monitored daily, were as follows: temperature 18.8 ± 0.2°C, pH 7.94±0.03, dissolved oxygen saturation 99.3% ± 1.1 and 16:8 hours of light:dark photoperiod. 90% of the water was renewed daily and EE2 and DMSO concentrations were adjusted after that.

2022-05-23 | PXD027547 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data