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Transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells

ABSTRACT: This study characterized transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells. Cells were transiently transfected with either GPNMB-targeting siRNA or a non-targeting control siRNA. RNA sequencing was performed using three independently prepared biological samples per group. Differential-expression and enrichment analyses were used to identify candidate biological processes and signaling pathways associated with GPNMB knockdown. Several pathways, including cAMP signaling, appeared among the nominal enrichment outputs. The cAMP pathway was therefore treated as a hypothesis-generating candidate for subsequent experimental investigation. The deposited transcriptomic dataset does not by itself establish an exclusive downstream signaling pathway, immune-suppressive function, clinical prognostic value, or therapeutic efficacy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE330529 | GEO | 2026/05/17

REPOSITORIES: GEO

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Transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells

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