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QMAP decodes RNA fragmentation dynamics

ABSTRACT: Recent advances in small RNA research reveal that noncanonical small RNAs, such as tRNA-derived small RNAs (tsRNAs) and rRNA-derived small RNAs (rsRNAs), are more abundant than well-known microRNAs in various tissue/cell types. Distinct fragmentation of parental RNAs (e.g., tRNA and rRNA) can generate functionally diverse small RNAs. Therefore, we propose a computational tool (qMAP) to identify differential fragmentation of parental RNAs between different biological conditions. Using qMAP, we tested aging-associated differential RNA fragmentation in mouse sperm heads between young and old mice. 28S rRNA was found to exhibit the most significant differential fragmentation. One short rsRNA candidate (17-nt) and one long rsRNA candidate (44-nt) were selected as their involvement of the observed differential fragmentation of 28S rRNA. These two candidate rsRNAs were transfected into mouse embryonic stem cells (mESCs), respectively. mRNA sequencing on these mESCs revealed a distinct transcriptomic response to the 17-nt and 44-nt rsRNAs.

ORGANISM(S): Mus musculus

PROVIDER: GSE330870 | GEO | 2026/05/13

REPOSITORIES: GEO

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