Single-Cell RNA Sequencing of TGF-Treated Human iPSC-Derived Skin Organoids

ABSTRACT: Human iPSC-derived skin organoids provide a powerful model for studying skin development and disease. Here, we investigated the effects of TGF-β treatment on human iPSC-derived skin organoids using single-cell RNA sequencing (scRNA-seq). Transcriptomic analysis identified diverse cellular populations, including keratinocytes and fibroblast-like cells, and revealed significant TGF-β-induced changes in cell states and gene expression. TGF-β treatment upregulated profibrotic and extracellular matrix-associated genes and activated pathways related to tissue remodeling and epithelial–mesenchymal transition. These findings demonstrate that iPSC-derived skin organoids recapitulate key features of TGF-β-mediated skin remodeling and provide a useful platform for studying fibrosis and cellular heterogeneity at single-cell resolution.

ORGANISM(S): Homo sapiens

PROVIDER: GSE330913 | GEO | 2026/06/10

REPOSITORIES: GEO