Transcriptomic profiling of rat supraspinatus muscle after tissue-adaptive flexible ultrasound patch treatment in a rotator cuff injury model

ABSTRACT: Rotator cuff tear is frequently accompanied by progressive supraspinatus muscle degeneration, including muscle atrophy, fibrosis, fatty infiltration and inflammatory remodeling. In this study, we performed bulk RNA sequencing to investigate the transcriptional effects of a tissue-adaptive flexible ultrasound patch (TA-FUP) on supraspinatus muscle degeneration after rotator cuff injury. A rat rotator cuff injury model was established by supraspinatus tenotomy combined with suprascapular neurotomy. Injured animals were assigned to untreated control or TA-FUP treatment groups. During treatment, the muscle-side region of TA-FUP was positioned over the supraspinatus muscle and operated at 100 mW/cm², while the tendon-side region was positioned over the tendon-to-bone interface and operated at 50 mW/cm². TA-FUP treatment was administered three times per week for 10 min per session. Bulk RNA-seq was performed on supraspinatus muscle tissues from control and TA-FUP-treated rats to characterize treatment-induced transcriptional changes. Differential expression and pathway analyses showed that TA-FUP broadly reprogrammed the degenerative transcriptomic profile of injured supraspinatus muscle. Genes and pathways associated with adipogenesis, lipid transport, lipid metabolic process, extracellular matrix organization, collagen fibril organization, immune response and inflammatory response were downregulated after TA-FUP treatment. Gene set enrichment analysis further suggested enhanced fatty acid degradation and suppression of adipocyte-associated lipid remodeling, ECM-receptor interaction and NF-κB signaling. These data provide transcriptomic evidence that TA-FUP attenuates supraspinatus muscle degeneration after rotator cuff injury by suppressing adipogenic, fibrotic and inflammatory remodeling programs.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE331125 | GEO | 2026/05/31

REPOSITORIES: GEO