Dataset Information

A malectin-like receptor kinase and bacterial motility shape tissue‑specific maize–Xanthomonas vasicola interactions

ABSTRACT: Understanding how pathogens infect different tissue types and how plants mount tissue-specific defenses against bacterial pathogens is critical for improving disease resistance. Xanthomonas vasicola pv. vasculorum (Xvv) is a bacterial pathogen of maize that exhibits both vascular and nonvascular lifestyles, offering a unique opportunity to dissect host-pathogen interactions across tissue types. Here, we combined fluorescence microscopy, dual RNA-seq, and functional genetics to characterize the interactions between Xvv and maize, both vascular and nonvascular. Using distinct inoculation methods, we reveal temporally and spatially divergent colonization patterns and transcriptional responses in resistant (B73) and susceptible (CML333) maize lines. We identify a role for a malectin-like receptor kinase (ZmCrRLK1L19) in vascular disease resistance using transposon insertion lines. The gene colocalizes with a major Xvv vascular resistance QTL. Xvv modulates motility and virulence gene expression depending on tissue context; flagellar motility is essential for mesophyll colonization but dispensable in the xylem. These findings reveal mechanisms underlying tissue-specific resistance and pathogen tissue tropism, establishing the maize–Xvv system as a model for studying dual-lifestyle bacterial pathogens.

ORGANISM(S): Zea mays Xanthomonas vasicola pv. vasculorum

PROVIDER: GSE331178 | GEO | 2026/05/20

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis; Jinrong Wan,1 Xuecheng Zhang,1 David Neece,2 Katrina M. Ramonell,3 Steve Clough,2,4 Sung-yong Kim,1 Minviluz Stacey,1 and Gary Stacey1*; 1Division of Plant Sciences, National Center for Soybean Biotechnology, C.S. Bond Life Sciences Center, University of Missouri-Columbia, Columbia, MO 65211, USA; 2Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; 3Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA; 4US Department of Agriculture, Soybean/Maize Germplasm, Pathology and Genetics Research, Urbana, IL 61801, USA; *To whom correspondence should be addressed. E-mail: staceyg@missouri.edu; Abstract: Chitin, a polymer of N-acetyl-D-glucosamine, is found in fungal cell walls, but not in plants. Plant cells are capable of perceiving chitin fragments (chitooligosaccharides) to trigger various defense responses. We identified a LysM receptor-like protein (AtLysM RLK1) that is required for the perception of chitooligosaccharides in Arabidopsis. Mutation of this gene blocked the induction of almost all chitooligosaccharide-responsive genes (CRGs) and led to more susceptibility to fungal pathogens, but not to a bacterial pathogen. In addition, exogenously applied chitooligosaccharides enhanced resistance against both fungal and bacterial pathogens in the wild-type plants, but not in the mutant. Together, our data strongly suggest AtLysM RLK1 is the chitin receptor or a key part of the receptor complex and chitin is a PAMP (pathogen-associated molecular pattern) in fungi recognized by the receptor leading to the induction of plant innate immunity against fungal pathogens. Since LysM RLKs were also recently shown to be critical for the perception of the rhizobial lipo-chitin Nod signals, our data suggest that LysM RLKs not just recognize friendly symbiotic rhizobia (via their lipo-chitin Nod signals), but also hostile fungal pathogens (via their cell wall chitin). These data suggest a possible evolutionary relationship between the perception mechanisms of Nod signals and chitin by plants. Experiment Overall Design: wild type Col-0 and chitin receptor mutants treated with or without chitooctaose

Dataset Information

A malectin-like receptor kinase and bacterial motility shape tissue‑specific maize–Xanthomonas vasicola interactions

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets