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Single-nucleus multiome RNA and ATAC sequencing of mouse kidney in kidney-specific Glut2 knockout (rGlut2 KO)

ABSTRACT: Glycosuria, whether genetically induced or triggered by SGLT2 inhibitors, activates compensatory glucose-producing pathways that limit glucose lowering in type 2 diabetes. To define these pathways, we studied renal Glut2 knockout mice, which progressively lose Slc5a2 (encoding SGLT2) expression yet maintain normoglycemia despite marked urinary glucose loss. Nuclei were isolated from three pooled flash-frozen kidney samples per group (12-week-old male rGlut2 KO and wild-type control mice) and single-nucleus gene expression and ATAC libraries were constructed using the 10x Chromium Multiome kit (Singulomics Corporation). Libraries were sequenced with ~200 million paired-end reads (PE150) on the Illumina NovaSeq. Reads were aligned to mm10 (2020-A) using Cell Ranger ARC 2.0.2. Downstream analysis used Seurat v5.1.0 and Signac v1.13.0.

ORGANISM(S): Mus musculus

PROVIDER: GSE331205 | GEO | 2026/07/16

REPOSITORIES: GEO

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