Transcriptomics,Genomics

Dataset Information

45

Impaired revascularization in a mouse model of diabetes associated with dysregulation of angiogenic-regulatory network.


ABSTRACT: Diabetes is a risk factor for the development of cardiovascular diseases that are associated with impaired angiogenesis or increased endothelial cell apoptosis. Here is it shown that angiogenic repair of ischemic hind limbs was impaired in Lepr db/db mice, a leptin receptor deficient model of diabetes, compared to wild-type C57BL/6 (WT) mice as evaluated by laser Doppler flow and capillary density analyses. To identify molecular targets associated with this disease process, hind limb cDNA expression profiles were created from adductor muscle of Lepr db/db and WT mice before and after hind limb ischemia using Affymetrix GeneChip® Mouse Expression Set microarrays. The expression patterns of numerous angiogenesis related proteins were altered in Lepr db/db versus WT mice following ischemic injury. These transcripts included neuropilin-1, VEGF-A, placental growth factor, elastin and matrix metalloproteinases that are implicated in blood vessel growth and maintenance of vessel wall integrity. These data illustrate that impaired ischemia-induced neovascularization in type 2 diabetes is associated with the dysregulation of a complex angiogenesis-regulatory network. Keywords: Diabetes, ischemia, angiogenesis, microarrays Overall design: Gene expression data for adductor muscle in the ischemic limb from wild type mice and Lepr db/db mice of 4 time points (before hind limb ischemia, 1 day, 7 days and 14 days after hind limb ischemia surgery). Three independent experimental replicates of each condition for each time point.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

SUBMITTER: Stephan Schiekofer  

PROVIDER: GSE3313 | GEO | 2014-06-30

SECONDARY ACCESSION(S): PRJNA93345

REPOSITORIES: GEO

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Impaired revascularization in a mouse model of type 2 diabetes is associated with dysregulation of a complex angiogenic-regulatory network.

Schiekofer Stephan S   Galasso Gennaro G   Sato Kaori K   Kraus Benjamin J BJ   Walsh Kenneth K  

Arteriosclerosis, thrombosis, and vascular biology 20050526 8


OBJECTIVE: Diabetes is a risk factor for the development of cardiovascular diseases associated with impaired angiogenesis or increased endothelial cell apoptosis. METHODS AND RESULTS: Here it is shown that angiogenic repair of ischemic hindlimbs was impaired in Lepr(db/db) mice, a leptin receptor-deficient model of diabetes, compared with wild-type (WT) C57BL/6 mice, as evaluated by laser Doppler flow and capillary density analyses. To identify molecular targets associated with this disease proc  ...[more]

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