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ICL002: Time course of Calu-3 cells infected with Influenza A/VN/1203/04

ABSTRACT: Purpose of experiment was to perform transcriptomics on Calu- 3 cells infected with Influenza A/VN/1203/04.

ORGANISM(S): Homo sapiens

PROVIDER: GSE33142 | GEO | 2011/11/01

SECONDARY ACCESSION(S): PRJNA149391

REPOSITORIES: GEO

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ICL002: Time course of Calu-3 cells infected with Influenza A/VN/1203/04

Project description:Purpose of experiment was to perform transcriptomics on Calu- 3 cells infected with Influenza A/VN/1203/04. Calu-3 cells were infected with Influenza A/VN/1203/04, at MOI of 1.0. Cells samples were collected at 0, 3, 7, 12, 18, and 24 h post infection. Each infected sample was done in duplicate. (Duplicates are defined as 2 different wells, plated at the same time using the same cell stock for all replicates.)There is one time-matched mock for each time point from the same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) was used for the mock infections. The NIAID Systems Virology Center

2011-11-01 | E-GEOD-33142 | biostudies-arrayexpress

IM001: Influenza A/VN/1203/04 infection of C57Bl/6 mouse model - Data from 3 viral doses at 1, 2, 4 and 7 days post infection

Project description:Purpose of experiment was to perform transcriptomic analysis on C57Bl/6 mice infected with different doses of Influenza A/VN/1203/04 at 4 different days post infection.

2011-11-01 | GSE33263 | GEO

IM001: Influenza A/VN/1203/04 infection of C57Bl/6 mouse model - Data from 3 viral doses at 1, 2, 4 and 7 days post infection

Project description:Purpose of experiment was to perform transcriptomic analysis on C57Bl/6 mice infected with different doses of Influenza A/VN/1203/04 at 4 different days post infection. Twenty-week-old C57BL/6 mice were infected by intranasal instillation of 10^2, 10^3 or 10^4 PFU of VN1203 in 50 µl of PBS or mock-infected with PBS alone. At days 1, 2, 4 and 7 days post-infection, lungs were harvested and total RNA was isolated and subjected to microarray analysis. The experimental design was for 4- 5 mice/time point/dose and 3 time-matched mocks. However due to survival rate, there are only 3 samples at Day 7 at 10^3 PFU and no day 7 samples at 10^4 PFU. The NIAID Systems Virology Center

2011-11-01 | E-GEOD-33263 | biostudies-arrayexpress

Host Regulatory Network Response in Calu-3 cell Infection with A/CA/04/2009 Influenza virus

Project description:Differential Expression was determined in Calu-3 cells between mock infected and infection with A/CA/04/2009 Influenza virus at nime time points post infection.

2012-05-05 | GSE37571 | GEO

Host Regulatory Network Response in Calu-3 cell Infection with A/CA/04/2009 Influenza virus

Project description:Differential Expression was determined in Calu-3 cells between mock infected and infection with A/CA/04/2009 Influenza virus at nime time points post infection. Calu-3 cells were infected with A/CA/04/2009 Influenza virus at MOI of 3, samples were collected 0,3,7,12,18, 24, 30, 36 and 48 hpi. Expression profiles and DE genes were determined for all time points. There are 3 mock and infected replicates for each time point.

2012-05-04 | E-GEOD-37571 | biostudies-arrayexpress

ICL0012 - Host response in human Calu-3 cells to infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1)

2013-01-05 | GSE43204 | GEO

H5N1 and X179A influenza infection of mice

Project description:Analysis of lung samples from mice infected with a severe H5N1 influenza virus (VN/1203/04/H5N1) or a mild H1N1 influenza virus (NYMC-X-179A) on day 3 and day 5 post-infection. Uninfected controls were used for comparison.

2016-12-20 | GSE70882 | GEO

ICL0012 - Host response in human Calu-3 cells to infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1)

Project description:Differential expression was determined in Calu-3 cells between mock infected and infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1) at different times post infection. Purpose: To obtain samples for transcriptional analysis in triplicate using the VN1203 pathogenicity mutant NS1-trunc124. Overview of Experiment: . Time Points: 0, 3, 7, 12, 18 and 24 hrs post infection. . Done in triplicate. . Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates. . Time-matched mocks were done in triplicate from the same cell stock as the rest of the samples. . Culture medium (the same as what the virus stock is in) was used for the mock infections. Calu-3 cells were infected with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or mock infected and samples were collected at 0, 3, 7, 12, 18 and 24 hpi. Calu-3 cells were infected with WT: A/Vietnam/1203/2004 (H5N1) and samples were collected at 7 and 24 hpi. There are 3 mock (2 included here) and 3 infected replicates for each time point. Expression profiles were determined.

2013-01-05 | E-GEOD-43204 | biostudies-arrayexpress

Human Calu-3 cell transcriptome response to wild-type pandemic H1N1 (A/California/04/2009), natural isolate

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type pandemic H1N1 (A/California/04/2009), natural isolate.

2016-05-07 | GSE80698 | GEO

Human Calu-3 cell transcriptome response to wild-type pandemic H1N1 (A/California/04/2009), natural isolate [mRNA]

2016-05-26 | GSE80697 | GEO

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