Genomics

Dataset Information

43

The role of dietary leucine and dairy protein on gastrocemius muscle gene expression


ABSTRACT: We used microarray to determine the differences in hepatic gene expression for diet-induced obese Sprague-Dawley rats consuming different dietary proteins. Proteins of interest included skim milk powder (dairy), casein, and the branched-chain amino acid, leucine. The primary aims of this study were: (i) to compare the effects of diets with protein derived from casein, casein supplemented with leucine, and complete dairy on body composition and insulin sensitivity; and (ii) to determine if there is a synergistic effect of dietary Ca and protein source on body composition and insulin sensitivity. Secondarily, we used microarray analysis to examine the effect of casein, leucine, or complete dairy containing diets on the expression of gastrocnemius muscle genes related to lipid and glucose metabolism. Overall design: Diet induced obese rats consumed ad libitum, a high fat, high sucrose diet for 8 weeks (n=4). All diets had an energy density of 4.6 kcal/gram and provided 10% of total energy from protein [casein, complete dairy (skim milk powder), or leucine-supplemented casein (7.1% from casein plus 2.9% from leucine)]. The casein treatment was the control diet.

INSTRUMENT(S): [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Lindsay K Eller  

PROVIDER: GSE33144 | GEO | 2011-10-22

SECONDARY ACCESSION(S): PRJNA149395

REPOSITORIES: GEO

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