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Genome-wide profiling of small RNAs in maize under abiotic stresses

ABSTRACT: To identify novel miRNA and NAT-siRNAs that are associated with abiotic stresses in maize, we generated small RNA sequences from maize seedlings that grew under control and under dought, salt, and cold stress treatments.

ORGANISM(S): Zea mays

PROVIDER: GSE33211 | GEO | 2015/10/21

SECONDARY ACCESSION(S): PRJNA149435

REPOSITORIES: GEO

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Genome-wide profiling of small RNAs in maize under abiotic stresses

Project description:To identify novel miRNA and NAT-siRNAs that are associated with abiotic stresses in maize, we generated small RNA sequences from maize seedlings that grew under control and under dought, salt, and cold stress treatments. Sequencing of small RNAs in maize under control, drought, salt, and cold stress conditions.

2015-10-21 | E-GEOD-33211 | biostudies-arrayexpress

Genome-wide profiling of small RNAs in sorghum under abiotic stresses

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Maize root LC-MSMS - Comparative proteomics of contrasting maize genotypes provides insights into salt-stress tolerance mechanisms

Project description:Salt stress is one major abiotic stress limiting maize grain yield throughout the world.To better understand maize salt tolerance molecular mechanism, comparative proteomic analysis was conducted on seedling roots of salt-tolerant genotype Jing724 and salt-sensitive genotype D9H under NaCl. Jing724 exhibited significantly higher germination rate and growth parameters (weight/length) than did D9H under salt treatment.We identified 565 differentially regulated proteins (DRP) usingiTRAQ and 89 were specific to Jing724 while 424 were specific to D9H. In salt stressed Jing724, pentose phosphate pathway, glutathione metabolism and nitrogen metabolism were enriched. By pathway enrichment and protein-protein interaction analyses, key DRPs such as glucose-6-phosphate 1-dehydrogenase, NADPH producing dehydrogenase, glutamate synthase and glutamine synthasewere identified.Additionally, salt responsive proteins of Jing724 were indicated to facilitate energy management, maintenance of redox homeostasis, reducing ammonia toxicity, osmotic homeostasis regulation, stress defense, stress adaptation, biotic cross-tolerance and gene transcription regulation. Quantification of multiple metabolic or enzymatic changes including SOD activity, MDA content, relative electrolyte leakage and Proline content were consistent with their predicted changes based on functions of DRPs. DRP analysis was correlated with the mRNA transcripts abundance variation of eight representative DRPs. These results contribute to elucidating molecular networks of salt tolerance.

2017-12-05 | PXD007711 | Pride

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