Genomics

Dataset Information

0

Toxicogenomic profiling of trout tumor promoters


ABSTRACT: Indole-3-carbinol (I3C), from cruciferous vegetables, has been found to suppress or enhance tumors in several animal models. We previously reported that dietary I3C promotes hepatocarcinogenesis in rainbow trout (Oncorhynchus mykiss) at concentrations that differentially activated estrogen receptor (ER) or aryl hydrocarbon receptor (AhR)-mediated responses based on individual protein biomarkers. In this study, we evaluated the relative importance of these pathways as potential mechanisms for I3C on a global scale. Hepatic gene expression profiles were examined in trout after dietary exposure to 500 and 1500 ppm I3C and 3,3,-diindolylmethane (DIM), a major in vivo component of I3C, and were compared to the transcriptional signatures of two model hepatic tumor promoters; 17beta-estradiol (E2), an ER agonist, and beta-naphthoflavone, an AhR agonist. We demonstrate that I3C and DIM acted similar to E2 at the transcriptional level based on correlation analysis of expression profiles and clustering of gene responses. Of the genes regulated by E2 (fold change >2.0 or <0.50), most genes were regulated similarly by DIM (87-92%) and I3C (71%) suggesting a common mechanism of action. Of interest were upregulated genes associated with signaling pathways for cell growth and proliferation, vitellogenesis, and protein folding, stability and transport. Other genes down-regulated by E2, including those involved in acute-phase immune response, were also down-regulated by DIM and I3C. Gene regulation was confirmed by qRT-PCR and western blot. These data indicate I3C promotes hepatocarcinogenesis through estrogenic mechanisms in trout liver and suggest DIM may be an even more potent hepatic tumor promoter in this model. Keywords: dose response

ORGANISM(S): Salmo salar Oncorhynchus mykiss

PROVIDER: GSE3324 | GEO | 2005/09/28

SECONDARY ACCESSION(S): PRJNA93221

REPOSITORIES: GEO

Similar Datasets

2006-12-31 | GSE5644 | GEO
2006-12-31 | GSE5643 | GEO
2023-10-16 | GSE232234 | GEO
2023-10-16 | GSE232233 | GEO
2023-10-16 | GSE232235 | GEO
| E-MTAB-4076 | biostudies-arrayexpress
2011-08-02 | GSE31085 | GEO
| E-GEOD-31085 | biostudies-arrayexpress
2015-05-08 | GSE57490 | GEO
| E-GEOD-7837 | biostudies-arrayexpress