Spatial transcriptomics profiling of NDMA-induced liver injury in Mgmt-deficient mice across sex and time
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ABSTRACT: Spatial transcriptomics (10x Genomics Visium) was performed on fresh-frozen liver tissue (left lobe) from Mgmt-/- mice treated with N-nitrosodimethylamine (NDMA) or saline vehicle during the juvenile period. The study includes male and female mice sacrificed at 10 weeks or 10 months post-birth to characterize spatially resolved transcriptomic changes associated with NDMA-induced liver damage and potential carcinogenesis over time. Mice carried the RaDR+/+; GPT+/+ genotype enabling detection of recombination and mutation events. Tissue sections were processed using the 10x Visium workflow, H&E stained, imaged, permeabilized for spatially barcoded cDNA synthesis, and sequenced on an Illumina NextSeq 500.
ORGANISM(S): Mus musculus
PROVIDER: GSE332601 | GEO | 2026/05/22
REPOSITORIES: GEO
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