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Alterations in chromatin accessibility during osteoblast and adipocyte differentiation in human mesenchymal stem cells

ABSTRACT: Although differential expression of genes is apparent during the adipogenic/osteogenic differentiation of marrow mesenchymal stem cells (MSCs), it is not known whether this is associated with changes in chromosomal structure. In this study, we used ATAC-sequencing technology to observe variations in chromatin assembly during the early stages of MSC differentiation. This showed significant changes in the number and distribution of chromosome accessibility at different time points of adipogenic/osteogenic differentiation. Sequencing of differential peaks indicated alterations in transcription factor motifs involved in MSC differentiation. We then integrated ATAC-seq and RNA-seq and found that only a small proportion of the overlapping genes were screened out from ATAC-seq and RNA-seq overlapping.

ORGANISM(S): Homo sapiens

PROVIDER: GSE332758 | GEO | 2026/05/28

REPOSITORIES: GEO

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