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Gene expression profiles of S. cerevisiae under heat stress

ABSTRACT: Genes whose expression correlated to the degree of thermotolerance in S. cerevisiae were identified by DNA microarray analysis.

ORGANISM(S): Saccharomyces cerevisiae Schizosaccharomyces pombe

PROVIDER: GSE33276 | GEO | 2012/08/30

SECONDARY ACCESSION(S): PRJNA149099

REPOSITORIES: GEO

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Gene expression profiles of S. cerevisiae under heat stress

Project description:Genes whose expression correlated to the degree of thermotolerance in S. cerevisiae were identified by DNA microarray analysis. Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared and a thermotolerance-related gene chosen.

2012-08-30 | E-GEOD-33276 | biostudies-arrayexpress

Gene expression profiles of S. cerevisiae under acetic acid stress

Project description:Genes whose expression correlated to the acetic acid tolerance in S. cerevisiae were identified by DNA microarray analysis.

2012-08-30 | GSE36914 | GEO

Gene expression profiles of S. cerevisiae under acetic acid stress

Project description:Genes whose expression correlated to the acetic acid tolerance in S. cerevisiae were identified by DNA microarray analysis. Gene expression profiles of two S. cerevisiae strains showing different levels of acetic acid tolerance were compared and an acetic acid tolerance-related gene chosen.

2012-08-30 | E-GEOD-36914 | biostudies-arrayexpress

Adaptive evolution under modest thermal stress improves the thermotolerance and ethanol fermentability of Saccharomyces cerevisiae

Project description:Thermotolerance development of robust Saccharomyces cerevisiae is necessary to enhance enzyme activity of cellulase, lower cooling costs, and reduce cell harm from the bad-distributed heat transfer in large-scale fermentation. The process-based studies of adaptive evolution have been well documented, but it remains unknown for the underlying molecular mechanism of the improved thermotolerance and the facilitated ethanol fermentability derived from adaptive evolution. Here, a robust thermotolerant S. cerevisiae Z100 was obtained with significantly improved ethanol fermentability under the stress of high temperature (50 oC) after 91 days’ adaptive evolution. RNA sequencing showed that adaptive evolution and its derived thermotolerance contributed to the unique gene transcriptional landscapes of the evolved strain. An interesting phenomenon was that the gene transcriptional signals of carbon metabolism were strengthened not at 50 oC but at 30 oC in S. cerevisiae Z100, and thus suggested that the improved thermotolerance led to the enhanced ethanol fermentability at 30 oC. The deeply repressed gene transcriptional expression indicated ribosome would be another key thermotolerant mechanism for the evolved strain. This study would provide a robust thermotolerant S. cerevisiae for bioethanol production and an important clue for future synthetic biology to thermotolerance engineering of fermentation strains.

2021-08-20 | GSE182342 | GEO

Expression data from Arabipdosis msh1 recA3 double mutant under heat stress

2010-03-31 | GSE19603 | GEO

Expression data from Arabipdosis msh1 recA3 double mutant under heat stress

Project description:In Arabidposis thaliana, the msh1 recA3 double mutant shows an extensive mitochondrial genome rearrangement and displays pronounced thermotolerance. We use microarray analysis to characterize genes and their response in the double mutant to a heat shock experiment in which plants were exposed to a temperature of 37 C for 2 hours. Wild type and double mutant plant were exposed to a temperature of 37 C for 2 hours and RNA samples were collected for the wild type and the double mutant before and after the heat shock.

2010-04-12 | E-GEOD-19603 | biostudies-arrayexpress

Phosphoproteome dynamics of Saccharomyces cerevisiae under heat shock and cold stress.

Project description: Not available

| S-EPMC4501848 | biostudies-other

S. cerevisiae subjected to prolonged heat stress of 37 ºC

Project description:Differential proteome composition of wild-type yeast cells (S. cerevisiae BY4741) grown at 30 ºC or 37 ºC was studied using SILAC based quantitative proteomics.

2015-04-23 | PXD001864 | Pride

Time-resolved heat stress response of Saccharomyces cerevisiae

Project description:Whole-genome transcriptional response of S. cerevisiae to an increase in temperature from 28°C to 41°C under well-controlled conditions. Two subsequent phases of response with very different dynamics: a short term response for the first hour after the temperature increase and a long term one for up to six hours. The initial response was strongest with almost half of the ORFs being induced or repressed to a statistically significant level (here 1.5 fold). The data was grouped based on the function of the encoded proteins. Analysis showed that the cells overexpressed genes involved in energy conservation processes. Genes encoding molecular chaperones were overexpressed as well, presumably to counteract the effect of the temperature increase on protein denaturation. Furthermore, genes encoding parts of the translation and transcription systems were repressed temporarily, in line with the observed lag in growth. More detailed analysis of certain small groups of genes involved in energy metabolism supported the notion that, although the expression level of genes represent a part of the stress response, they cannot be directly linked to the level of activity of their products.

2013-12-01 | GSE25503 | GEO

Dissecting the yeast heat shock response by CRISPRi/a

Project description:CRISPRi screens on the repression of 129 protein kinases and 161 transcription factors in S. cerevisiae. We quantify perturbation effects on cellular fitness at 23, 30 and 38°C, expression of the SSA1 Hsp70 chaperone (as proxy for heat shock response activity) and thermotolerance. The integration of these phenotypes allowed us to identify core signaling pathways of the HSR and their contributions to temperature-associated growth and heat resistance.

2021-07-01 | GSE155455 | GEO

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