H4K16ac contributes to chromatin compartment reorganization during mitotic and meiotic transitions [Hi-C 2a]

ABSTRACT: Chromatin compartments reorganize dramatically during cell cycle transitions, shifting from a compartmentalized interphase organization to a condensed mitotic configuration in which large-scale compartment signals are attenuated. While histone modifications are linked to compartment identity, their causal contribution to compartment organization remained unclear. Using meiotic (mouse spermatogenesis) and mitotic (mouse neuronal progenitor cells, mNPCs) models, we mapped histone landscapes alongside compartment dynamics. We found that histone H4 lysine 16 acetylation (H4K16ac) strongly tracked compartment reorganization, remaining enriched in A compartments during compartmentalization but redistributing upon chromatin compaction. In spermatogenesis, H4K16ac loss on the X chromosome coincided with condensation and H3K9me3 spreading. Machine learning highlighted H4K16ac as a stronger predictor of compartment transitions than H3K27ac. To probe causality, we acutely degraded MOF, the principal H4K16ac acetyltransferase, which disrupted both the spatial distribution and global abundance of H4K16ac and progressively impaired compartmentalization in mNPCs. Together, these results position H4K16ac as a selectively coupled and influential histone modification of chromatin compartment transitions.

ORGANISM(S): Mus musculus

PROVIDER: GSE332823 | GEO | 2026/05/27

REPOSITORIES: GEO