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Comparison of iPSC lines during differentiation to intermediate mesoderm

ABSTRACT: To examine variability in differentiation outcomes, distinct iPCS lines were differentiated to intermediate mesoderm following the protocol of Takasato et al (2015). Cells were treated with 4 or 5 days of CHIR followed by 4 days of FGF9, dissociated and profiled to generate bulk transcriptome data for each. Cell lines used were "MAFB-mtagBFP;GATA3-mCherry" described in Vanslambrouck et al, JASN, 2019; "NG-hiPSC line1B", D2MCBL01B, described in Novoa et al, Cytotherapy 2024; AICS-0061, "H2B-GFP AICS", a H2B-GFP reporter from the Allen Institute of Cell Science, Allen Cell Collection.

ORGANISM(S): Homo sapiens

PROVIDER: GSE333258 | GEO | 2026/07/09

REPOSITORIES: GEO

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