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Trans-kingdom, rapid, affordable Purification of RISCs (TraPR) enrichment of small RNAs from Cuscuta campestris in vitro haustoria and flanking host stems of Arabidopsis thaliana

ABSTRACT: The obligate parasitic plant Cuscuta campestris delivers trans-species microRNAs (miRNAs) into host plants to silence host mRNAs. Here, the genetic requirements for biogenesis, movement, and function of these miRNAs were investigated. Primary miRNA transcript accumulation precedes mature miRNA accumulation by 24 to 48 hours. Trans-species miRNAs accumulate in host tissues a short distance from the site of parasite attachment. Trans-species miRNAs require C. campestris but not host Dicer-Like 1 (DCL1) for accumulation. These miRNAs specifically avoid Argonaute (AGO) loading in C. campestris tissue where they instead accumulate as miRNA/miRNA* duplexes. After arrival and short-distance spreading in host tissues, they are loaded onto host AGO proteins including AGO1 and AGO2. This study clarifies the transcription, dicing, delivery, and function of C. campestris trans-species miRNAs. We propose that selective avoidance of self AGO-loading is a mechanism to facilitate high rates of delivery of these "export only" miRNAs to host tissues.

ORGANISM(S): Cuscuta campestris Arabidopsis thaliana

PROVIDER: GSE333470 | GEO | 2026/05/29

REPOSITORIES: GEO

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