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Systematic dissection of regulatory motifs in 2,000 predicted human enhancers using a massively parallel reporter assay

ABSTRACT: We employ a massively parallel reporter assay (MPRA) to measure the ex vivo activities of hundreds of K562 and HepG2 enhancers with known transcription factor motif instances. For seven selected motifs that correspond to known or predicted activators and repressors in the two cell types, we make directed modifications of the bases corresponding to these motifs and observe the changes in enhancer activity.

ORGANISM(S): Escherichia coli Homo sapiens

PROVIDER: GSE33367 | GEO | 2013/03/15

SECONDARY ACCESSION(S): PRJNA149003

REPOSITORIES: GEO

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Systematic dissection of regulatory motifs in 2,000 predicted human enhancers using a massively parallel reporter assay

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