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Insights into the impact of archaeal proteasome activity on cell division: CCTF1 plays a decisive role in regulating cellular proteasome activity [aCcrK OE]

ABSTRACT: The cell cycle is a precisely coordinated series of events of cell growth, DNA replication and cell division, all of which are imperative for the proliferation of organisms. Archaea of the order Sulfolobales exhibit a cell cycle similar to that of eukaryotes. It has been suggested that the proteasome degradation of the cell division protein CdvB controls the progression of cell division. This raises a question regarding the regulation of proteosome activity in these archaea. In this paper, we report that an ArsR family cyclically-transcribed cell cycle transcription factor, CCTF1 (cell cycle transcription factor 1), plays a decisive role in regulation of the proteasome activity. We revealed that transcription of the proteasome regulatory subunit PAN (proteasome-activating nucleotidase) is specifically regulated by CCTF1 through binding to an AT-rich palindromic sequence. We show that CCTF1, rather than aCcrK (ePK2), is the key factor controlling the proteasome activity. Our study provides important insights into the proteosome-mediated cell cycle regulation mechanism in archaea.

ORGANISM(S): Saccharolobus islandicus REY15A

PROVIDER: GSE333795 | GEO | 2026/08/01

REPOSITORIES: GEO

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Insights into the impact of archaeal proteasome activity on cell division: CCTF1 plays a decisive role in regulating cellular proteasome activity [CCTF1 OE]

Project description:The cell cycle is a precisely coordinated series of events of cell growth, DNA replication and cell division, all of which are imperative for the proliferation of organisms. Archaea of the order Sulfolobales exhibit a cell cycle similar to that of eukaryotes. It has been suggested that the proteasome degradation of the cell division protein CdvB controls the progression of cell division. This raises a question regarding the regulation of proteosome activity in these archaea. In this paper, we report that an ArsR family cyclically-transcribed cell cycle transcription factor, CCTF1 (cell cycle transcription factor 1), plays a decisive role in regulation of the proteasome activity. We revealed that transcription of the proteasome regulatory subunit PAN (proteasome-activating nucleotidase) is specifically regulated by CCTF1 through binding to an AT-rich palindromic sequence. We show that CCTF1, rather than aCcrK (ePK2), is the key factor controlling the proteasome activity. Our study provides important insights into the proteosome-mediated cell cycle regulation mechanism in archaea.

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