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Transcriptional Response to WNT-inhibition-induced human cardiomyogenesis (single-cell RNA-seq)

ABSTRACT: Since embryonic cardiac development in vivo and in vitro cardiomyocyte differentiation protocols both require WNT inhibition, our study focussed on how inhibition of WNT signalling guides the transcriptional landscape towards human cardiomyocyte differentiation. We uncovered transcriptional responses using single-cell RNA sequencing. with bioinformatics analysis defining cell identities, reconstructing differentiation trajectories, dissecting WNT inhibition-dependent gene expression and inferring Gene Regulatory Networks (GRN) driving cardiomyocyte specification. We found that WNT inhibition (WNTi) redirects early mesoderm precursors towards a cardiomyocyte fate while limiting alternative lineages.

ORGANISM(S): Homo sapiens

PROVIDER: GSE333970 | GEO | 2026/06/08

REPOSITORIES: GEO

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