Dataset Information

Topical Vascular Organoid Therapy Promotes Microvascular Regeneration and Functional Recovery in Porcine Ischemic Cardiomyopathy

ABSTRACT: Background: Ischemic heart disease (IHD) remains a leading cause of morbidity and mortality worldwide, with microvascular dysfunction playing a key role in the progression to ischemic heart failure.　Current revascularization strategies restore epicardial blood flow but fail to address impaired microcirculation. To overcome this limitation, we developed a scaffold-free vascular organoid therapy.Methods: An IHD pig model was created by occluding the left anterior descending artery. Bilayer vascular organoids (VOs) composed of human endothelial progenitor cells and mesenchymal stem cell–derived smooth muscle cells were transplanted onto the left ventricular 14 days after infarction. Animals were followed for four weeks with cardiac MRI and histological analyses.Results: VO transplantation preserved left ventricular ejection fraction, modestly improved regional function, and enhanced microvascular density and maturation. Transplanted cells migrated into host myocardium, and angiogenesis-related genes were upregulated.Conclusions: Topically transplanted VOs promote microvascular regeneration and help preserve cardiac function after ischemic injury.

ORGANISM(S): Sus scrofa

PROVIDER: GSE333973 | GEO | 2026/07/02

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Background: Coronary microvascular disease (CMD) is an early complication of type 2 diabetes (T2D) involving adverse endothelial and smooth muscle dysfunction, vascular remodeling, and alterations in mechanics, which culminate in impaired coronary blood flow. Recent data suggest that vascular layer-specific mechanisms, influenced by the surrounding myocardium, contribute to CMD. To investigate transcriptional differences potentially contributing to CMD, we used spatial and single cell transcriptomic profiling of the coronary microcirculation and surrounding myocardium across distinct heart subregions to identify new pathways to target. Methods: We combined single cell RNA profiling and spatial transcriptomics to examine transcriptional differences and molecular signatures of CMD in T2D mice. Results: Single cell RNA profiling and spatial transcriptomics revealed upregulation of genes linked to adipogenesis, fatty acid metabolism, and oxidative phosphorylation in T2D cell clusters and coronary microvascular-enriched regions. Using spatial transcriptomics, we also examined gene expression in coronary microvessel-enriched areas across different heart subregions. Interestingly, the most pronounced transcriptional differences between T2D and control coronary microvessels were observed in the left ventricular anterior wall, where inflammatory response pathways were downregulated in T2D mice. Genes associated with oxidative phosphorylation and fatty acid oxidation were significantly upregulated in T2D coronary microvessels within the left ventricular anterior wall.Conclusions: These intriguing data support the well-documented concept that cardiac metabolic inflexibility in T2D – characterized by reduced mitochondrial function, increased reliance on fatty acid oxidation, and impaired glucose utilization – contributes to oxidative stress and lipotoxicity, key drivers of heart failure. Furthermore, these data reveal previously unrecognized transcriptomic differences in coronary microvessels across spatially-distinct regions of the T2D heart, suggesting transcriptional heterogeneity in this critical vascular bed.

Dataset Information

Topical Vascular Organoid Therapy Promotes Microvascular Regeneration and Functional Recovery in Porcine Ischemic Cardiomyopathy

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets