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Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation

ABSTRACT: Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was submitted to 4 experimental treatments (C. sativus (spot blotch), P. hordei (leaf rust), and water and oil controls) to examine gene transcription differences triggered by biotrophic and hemi-biotrophic pathogens. Inoculated plants were arranged in a split plot design. Samples were collected at 12, 24, 36, & 48 hours after inoculation. A total of 48 samples (4 treatments x 4 time points x 3 replicates) were subjected to GeneChip analysis. Made public: 2009-12-02 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Millett. The equivalent experiment is BB61 at PLEXdb.]

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE33401 | GEO | 2011/11/22

SECONDARY ACCESSION(S): PRJNA149143

REPOSITORIES: GEO

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Transcriptome analysis of wild barley (H. vulgare ssp spontaneum) after pathogen inoculation

Project description:Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was submitted to 4 experimental treatments (C. sativus (spot blotch), P. hordei (leaf rust), and water and oil controls) to examine gene transcription differences triggered by biotrophic and hemi-biotrophic pathogens. Inoculated plants were arranged in a split plot design. Samples were collected at 12, 24, 36, & 48 hours after inoculation. A total of 48 samples (4 treatments x 4 time points x 3 replicates) were subjected to GeneChip analysis. Made public: 2009-12-02 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Millett. The equivalent experiment is BB61 at PLEXdb.] treated or untreated: C. sativus (spot blotch) - time: 12 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 24 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 36 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 48 hrs(3-replications); treated or untreated: water control - time: 12 hrs(3-replications); treated or untreated: water control - time: 24 hrs(3-replications); treated or untreated: water control - time: 36 hrs(3-replications); treated or untreated: water control - time: 48 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 12 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 24 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 36 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 48 hrs(3-replications); treated or untreated: oil control - time: 12 hrs(3-replications); treated or untreated: oil control - time: 24 hrs(3-replications); treated or untreated: oil control - time: 36 hrs(3-replications); treated or untreated: oil control - time: 48 hrs(3-replications)

2011-11-22 | E-GEOD-33401 | biostudies-arrayexpress

Short and long term cold responses in a winter barley

2011-03-08 | GSE27821 | GEO

Penetration resistance: Wildtype and ataf1-1 mutant response to Bgh 12 h after inoculation, 2*2 factorial design

2008-09-20 | GSE12856 | GEO

Long term cold responses in a winter barley

Project description:Gene expression changes maintained after vernalization ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Trevaskis. The equivalent experiment is BB95 at PLEXdb.]

2011-03-08 | GSE27822 | GEO

Comprehensive molecular characterization of the soybean rust disease [Embrapa-Soja - Iowa State Univ; 2005]

Project description:Experimental design: 2 genotypes: PI230970 (resistant USDA Plant Introduction (PI) line containing SBR Rpp2 resistance gene) & Embrapa-48 (susceptible Brazilian cultivar) 2 treatments: Soybean rust challenge & mock infection 3 replications 10 time points: 6, 12, 18, 24, 36, 48, 72, 96, 120, 168hai TOTAL: 120 Affymetrix GeneChip(R) Soybean Genome Arrays ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel. The equivalent experiment is GM2 at PLEXdb.]

2011-11-22 | GSE33410 | GEO

Short and long term cold responses in a winter barley

Project description:Gene expression changes in a winter cultivar of barley in response to short and long term cold treatments ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Trevaskis. The equivalent experiment is BB94 at PLEXdb.] treatment: Non-vernalized (3-replications); treatment: Short-term cold (3-replications); treatment: Vernalized (3-replications); treatment: 1 day post-vernalized (3-replications)

2011-03-08 | E-GEOD-27821 | biostudies-arrayexpress

Long term cold responses in a winter barley

2011-03-08 | E-GEOD-27822 | biostudies-arrayexpress

Transcription profiling of Arabidopsis penetration resistance, wildtype and ataf1-1 mutant response to Bgh 12 h after inoculation, 2x2 factorial design

Project description:Arabidopsis is a non-host to the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh), effectively hindering Bgh ingress to the epidermal cells by deposition of callosic papillae formations, i.e. penetration resistance. To better understand the transcriptional changes in Arabidopsis towards Bgh, we compared un-inoculated and inoculated wild-type Arabidopsis samples, with those an ATAF1 mutant allele, compromised in penetration resistance. The experiment included sampling of 8 rosettes for each replicate sample from 6-weeks old plants, 12 hrs after inoculation. A total of 12 biolocigal replicates were sampled with three replicates from each of the four blocks (Wild-type, ataf1-1, Bgh, control). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Michael Krogh Jensen. The equivalent experiment is AT31 at PLEXdb.] Experiment Overall Design: genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications)

2008-10-25 | E-GEOD-12856 | biostudies-arrayexpress

Barley host response to the direct application of the trichothecene mycotoxin deoxynivalenol

Project description:Barley florets (cv. Morex) were treated with 2.0 microgram deoxynivalenol per floret via a 10 microliter solution or mock inoculated with water. Samples were collected at 1, 12, 24, and 48 hours after inoculation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Stephanie A. Gardiner. The equivalent experiment is BB62 at PLEXdb.]

2011-11-22 | GSE33405 | GEO

Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation

Project description:Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Aravind Jukanti. The equivalent experiment is BB53 at PLEXdb.]

2011-11-22 | GSE33413 | GEO

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