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ECM1/SQSTM1 Axis Remodels Colorectal Cancer Immune Microenvironment via CXCL1-CXCR2-Dependent Neutrophil Recruitment and NETosis (Spatial transcriptomics)

ABSTRACT: Extracellular matrix protein 1 (ECM1) has been implicated in tumorigenesis; however, its precise role in modulating the immunosuppressive tumor microenvironment (ITM) in colorectal cancer (CRC) remains inadequately elucidated.

ORGANISM(S): Mus musculus

PROVIDER: GSE334234 | GEO | 2026/06/08

REPOSITORIES: GEO

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ECM1/SQSTM1 Axis Remodels Colorectal Cancer Immune Microenvironment via CXCL1-CXCR2-Dependent Neutrophil Recruitment and NETosis (scRNA-seq)

Project description:Extracellular matrix protein 1 (ECM1) has been implicated in tumorigenesis; however, its precise role in modulating the immunosuppressive tumor microenvironment (ITM) in colorectal cancer (CRC) remains inadequately elucidated.

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ECM1/SQSTM1 Axis Remodels Colorectal Cancer Immune Microenvironment via CXCL1-CXCR2-Dependent Neutrophil Recruitment and NETosis

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Transcriptome Sequencing Analysis of Human Colorectal Cancer Cell Lines After ECM1 Knockdown

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Human melanoma cells over-express Extracellular Matrix 1 (ECM1) which is regulated in part by TFAP2C

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Project description:Extracellular Matrix Protein-1 (ECM1) promotes tumorigenesis in multiple organs, but the mechanisms associated with the functions and putative receptors of ECM1 subtypes have yet to be systematically clarified. We found in this study that secretory ECM1a functions as an oncoprotein to induce tumorigenesis through binding of the Gly-Pro-Arg (GPR) motif to integrin aXb2 and activation of AKT/FAK/Rho/cytoskeletal signaling. Nonsecretory ECM1b binds to myosin to block myosin phosphorylation, thus preventing cytoskeletal signaling activation and tumorigenesis. Based on RNA sequencing, we further found that the heterogeneous nuclear ribonucleoprotein L-like (hnRNPLL) protein induced by ECM1a favors the alternative mRNA splicing of ECM1a to control ECM1-associated signaling and tumorigenesis. ATP binding cassette subfamily G member 1 (ABCG1) transduces ECM1a-integrin aXb2 interactive signaling to facilitate the phosphorylation of AKT/FAK/Rho/cytoskeletal molecules and to confer cisplatin resistance in cancer cells through upregulation of CD326-mediated cell stemness.

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