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Microglia integrated neural spheroids enable neuroinflammatory responses and correct network dysfunction induced by alpha-synuclein mutation.

ABSTRACT: Microglia are the primary resident immune cells of the brain, playing both protective and deleterious roles in neurological diseases, which make them an enticing therapeutic target. Here we developed a Neural Microglia Integrated Multicellular iPSC-derived Cultured Spheroids (NeuroMIMICS) system that enables measurements of both neural network activity and immune responses using human iPSC-derived microglia, astrocytes, and neurons. We validated microglia functionalities in these neural tri-cultures including phagocytic activity, directed motility, and inflammatory responses. RNAseq analysis revealed a phenotypical acquisition of immune functionality via microglia incorporation, supported by increased cytokine production in spheroids challenged with pathogen-like insults.

ORGANISM(S): Homo sapiens

PROVIDER: GSE335200 | GEO | 2026/07/17

REPOSITORIES: GEO

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