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R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions

ABSTRACT: Transcriptional profiling of R. sphaeroides Δirr under iron limitation (-Fe) compared to control R. sphaeroides Δirr under normal growth conditions (+Fe).

ORGANISM(S): Cereibacter sphaeroides 2.4.1

PROVIDER: GSE33533 | GEO | 2011/11/09

SECONDARY ACCESSION(S): PRJNA154271

REPOSITORIES: GEO

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R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides Δirr under iron limitation (-Fe) compared to control R. sphaeroides Δirr under normal growth conditions (+Fe). Two strain experiment under normal iron (+Fe) and iron limitation (-Fe) conditions. 6 Biological replicates, independently grown and harvested at OD660=0,4; 1-3 pooled in replicate 1, 4-6 pooled in replicate 2

2011-11-09 | E-GEOD-33533 | biostudies-arrayexpress

R. sphaeroides Δirr vs. R. sphaeroides 2.4.1 under microaerobic conditions

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2011-11-09 | GSE33534 | GEO

R. sphaeroides Δirr vs. R. sphaeroides 2.4.1 under microaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides Δirr compared to control R. sphaeroides 2.4.1 under microaerobic conditions. Two strain experiment under microarobic conditions (30 µM). 6 Biological replicas, independetly grown and harvested at OD660=0,4; 1-3 pooled in replicate 1, 4-6 pooled in replicate 2

2011-11-09 | E-GEOD-33534 | biostudies-arrayexpress

R. sphaeroides delta-cryB vs. R. sphaeroides 2.4.1 under blue light, semiaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides delta-cryB compared to control R. sphaeroides 2.4.1 under blue light, semiaerobic conditions.

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R. sphaeroides pRKPcrZ vs. R. sphaeroides pRK4352 under low oxygen conditions

Project description:Transcriptional profiling of R. sphaeroides pRKPcrZ compared to control R. sphaeroides pRK4352 under low oxygen conditions. In this study we compared the transcriptome of a PcrZ over-expression strain to a control strain. PcrZ is the first small non-coding RNA, which is involved in the complex regulatory network of photosynthesis gene regulation in Rhodobacter sphaeroides. It counteracts the formation of photosynthetic complexes in a redox dependent manner.

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Transcriptional profiling of R. sphaeroides Δirr

Project description:This SuperSeries is composed of the following subset Series: GSE33533: R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions GSE33534: R. sphaeroides Δirr vs. R. sphaeroides 2.4.1 under microaerobic conditions Refer to individual Series

2011-11-09 | E-GEOD-33535 | biostudies-arrayexpress

R. sphaeroides Δlov vs. R. sphaeroides 2.4.1 (microarobic conditions)

Project description:Transcriptional profiling of R. sphaeroides Δlov comparing to control R. sphaeroides 2.4.1 under microarobic conditions

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Transcriptome analysis and 5‘-end mapping of total RNA from Rhodobacter sphaeroides wild type and RNase E (rne) mutant strain, grown under aerobic, microaerobic or phototrophic conditions

Project description:The conserved endoribonuclease RNase E is essential in Rhodobacter sphaeroides and acts as global regulator of the transcriptome. By comparison of an RNase E mutant (showing reduced enzyme activity) with the Rhodobacter sphaeroides wild type, both grown under three different growth conditions, we analysed the impact of RNase E on the adaption of Rhodobacter sphaeroides to different growth conditions.

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R. sphaeroides delta-CryB vs. R. sphaeroides 2.4.1 microarobic conditions

Project description:Transcriptional profiling of R. sphaeroides delta-CryB comparing to control R. sphaeroides 2.4.1 under microaerobic conditions

2011-11-09 | GSE33554 | GEO

IscR of Rhodobacter sphaeroides functions as repressor of genes for iron-sulphur metabolism and represents a new type of iron-sulphur binding protein

Project description:Iron-sulphur (Fe-S) clusters are ensembles of iron and sulphide centres. They are found in all life forms and are important components of many enzymes involved in diverse cellular processes, including respiration, DNA synthesis or gene regulation. However, the increase in oxygen after the emergence of oxygenic photosynthesis created a threat to Fe–S proteins and, consequently, to the organisms relying on them. Therefore, bacteria have evolved mechanisms to maintain a precise intracellular iron concentration. A major role of IscR in R. sphaeroides iron dependent regulation was suggested in a bioinformatic study , which predicted a binding site in the upstream regions of several iron uptake genes. Most known IscR proteins have Fe-S clusters featuring (Cys)3(His)1 ligation. However, IscR proteins from Rhodobacteraceae harbour only one Cys residue and it was considered unlikely that they can ligate an Fe-S cluster. In this study, the role of R. sphaeroides IscR as transcriptional regulator and sensor of the Fe-S cluster status of the cell was analysed. The results provide evidence that R. sphaeroides IscR functions as transcriptional repressor of genes involved in iron metabolism by binding to the predicted DNA binding motif. Furthermore, IscR possesses a unique Fe-S cluster ligation scheme with only a single cysteine involved.

2015-02-03 | GSE65537 | GEO

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