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HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

ABSTRACT: The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes Keywords: repeat sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE336 | GEO | 2003/03/11

SECONDARY ACCESSION(S): PRJNA84811

REPOSITORIES: GEO

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HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes

2003-03-10 | E-GEOD-336 | biostudies-arrayexpress

cDNA array 6800 genes (NIB) - HaCaT J10 24h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 24 h after irradiation. Keywords = human keratinocytes, gamma irrdiation Keywords: dose response

2003-03-26 | GSE356 | GEO

cDNA array 6800 genes (NIB) - HaCaT J10 3h - 0.5&2 Gy

Project description:Huamn HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response

2003-03-13 | GSE338 | GEO

cDNA array 2304 genes (OSPE) - HaCaT J10 3h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response

2003-03-11 | GSE337 | GEO

cDNA array 6800 genes (NIB) - HaCaT J10 3h - 0.5&2 Gy

2003-03-13 | E-GEOD-338 | biostudies-arrayexpress

cDNA array 2304 genes (OSPE) - HaCaT J10 3h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation

2010-06-05 | E-GEOD-337 | biostudies-arrayexpress

cDNA array 6800 genes (NIB) - HaCaT J10 24h - 0.5&2 Gy

2003-03-25 | E-GEOD-356 | biostudies-arrayexpress

Homo sapiens

Project description:HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

| PRJNA84811 | ENA

Protein dataset of immortalized HaCaT cells p53 knock down HaCaT keratinocytes

Project description:The p53 protein is encoded by TP53 gene and plays the key role in significant number of cellular processes including proliferation, apoptosis and regulation of many stress response pathways. P53 acts like a direct transcription activator of numerous genes regulating cell cycle arrest, DNA repair, growth inhibition and many others (Mollereau and Ma, 2014). The canonical biological function of p53 is maintaining genome integrity via elimination of damaged or exposed to genotoxic stress cells. Immortalized HaCaT cells are widely used for keratinocyte research, since they maintain stable keratinocyte phenotype, have nearly unlimited proliferative potential, do not require specific growth and differentiation factors (Colombo et al., 2017). Also, HaCaT cells produce typical differentiation markers such as cytokeratins K14 and K10, involucrin (Colombo et al., 2017) and respond to keratinocyte differentiation stimuli. Taking together, HaCaT cells have similar to normal human keratinocytes (NHK) properties, however, as many of spontaneously immortalized cell lines HaCaT cells bear two mutant p53 alleles - R282Q and H179Y (Lehman et al., 1993). Mutp53 in HaCaT has an increased affinity to other p53 family members (p63, p73), which significantly expands p53 properties. Moreover, mutp53 indirectly affects specific target genes via protein-protein interactions with other transcription factors (NF-Y, E2F1, NF-KB) or by tethering p63 to new promotor locations. For more detailed investigation of mutp53 impact on various processes in HaCaT cells we performed a shRNA mediated knockdown of mutp53. For generation of stable TP53 knockdown we employed plasmid vector pLKO-p53-shRNA-941 (Addgene # #25637) followed by puromycin selection of transduced cells. Here we present proteomic dataset obtained from wild type HaCaT cells and p53 knock down HaCaT keratinocytes.

2022-04-28 | PXD033538 | Pride

Human keratinocytes HaCaT LC-MSMS_SDS exposure

Project description:We employed human HaCaT cells as a model system to identify cellular proteins that accompany SDS-induced toxicity based on a proteomic approach. HaCaT human keratinocyte cell line were treated with a non-cytotoxic dose of SDS (25 µg/ml, as determined by the MTT assay and microscopically examination) for 48 h. The altered abundance of proteins from HaCaT keratinocytes exposed to SDS was analyzed by LC-MS/MS approach and quantified using Progenesis LC software. The abundance of 217 proteins (which were identified by multiple peptides, ≥ 2) was altered in keratinocytes exposed to SDS; in which 131 proteins had increased abundance while 86 proteins was down regulated. The Pathview map of 131 up-regulated proteins was built and enhancement of glycolysis/gluconeogenesis was found.

2017-11-03 | PXD007789 | Pride

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