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Small RNA seq: POLR3G and POLR3GL knockout in THP-1 and POLR3G knockdown in HEK293T

ABSTRACT: RNA polymerase III (Pol III) synthesizes noncoding RNAs essential for translation, RNA processing, and transcription regulation. Its activity aligns with cellular growth and nutrient availability, and its dysregulation is linked to diseases like breast cancer and leukodystrophy. Here we explot the effect of POLR3G and POLR3GL knockout in THP-1 and POLR3G knockdown in HEK293T to small RNA expression

ORGANISM(S): Homo sapiens

PROVIDER: GSE336809 | GEO | 2026/07/09

REPOSITORIES: GEO

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FastGro-seq: POLR3G and POLR3GL knockout and HEK293T cell treated with Pol III inhibitor ML60218

Project description:RNA polymerase III (Pol III) synthesizes noncoding RNAs essential for translation, RNA processing, and transcription regulation. Its activity aligns with cellular growth and nutrient availability, and its dysregulation is linked to diseases like breast cancer and leukodystrophy. RNA polymerase III composition is shaped by the mutually exclusive incorporation of two paralogous subunits, RPC7α and RPC7β, encoded by genes POLR3G and POLR3GL in vertebrates. The expression of POLR3G and POLR3GL is spatiotemporally regulated during development, and multiple reports point to RPC7α-enhanced Pol III activity patterns, indicating that Pol III identity may underlie dynamic Pol III transcription patterns observed in higher eukaryotes. In cancer, upregulation of POLR3G, but not POLR3GL, is associated with poor survival outcomes among patients, suggesting differences between RPC7α and RPC7β further influence disease progression and may translate into future biomarkers and therapeutic strategies.

2026-07-10 | GSE337522 | GEO

POLR3G and POLR3GL-RNA polymerase III target genes

2013-10-01 | GSE47849 | GEO

POLR3G and POLR3GL-RNA polymerase III target genes

Project description:Purpose: Characterization of the target genes of two versions of the Polymerase III (pol III), one containing POLR3G subunit or POLR3GL subunit, in different tissues. Methods: We performed ChIP_seq of POLR3D and the two variants subunits, POLR3G and POLR3GL in Human IMR90 cells, Mouse liver and Mouse Hepatocarcinoma Hepa 1-6 cells in order to locate and analyse total pol III, POLR3G and POLR3GL-containing pol III in various tissues. Results: We show that POLR3G- as well as POLR3GL-containing pol III are present in cultured cell lines and in normal mouse liver, although the relative amounts of the two forms vary, with the POLR3G-containing pol III relatively more abundant in dividing cells. Both forms of pol III occupy the same target genes, in very constant proportions within one cell line, suggesting that the two forms of pol III have similar function with regard to specificity for target genes. Conclusions: POLR3G and POLR3GL occupy the same target genes, but they are differentially expressed under different conditions and in different cell types. Pol III target genes in human IMR cells, Mouse liver, and Mouse Hepatocarcinoma cells were identified by chromatin Immuno-precipitation followed by deep sequencing using Illumina HiSeq. For Human IMR90 cells, we used antibodies against POLR3D, POLR3G, POLR3GL and BDP1. For Mouse data, we targeted POLR3D, POLR3G and POLR3GL and did ChIP_seq replicates. We also sequenced the corresponding inputs (crosslinked DNA of IMR90 cells, Mouse liver, and Mouse Hepatocarcinoma cells). This represents a total of 20 samples.

2013-10-01 | E-GEOD-47849 | biostudies-arrayexpress

Genome-wide maps of RNA Pol III subunits POLR3C, POLR3G, and PLOR3GL in ES-E14TG2a and primary MEF cells

Project description:Mammalian cells contain two isoforms of RNA Polymerase III (Pol III) that differ in only a single subunit, with POLR3G in one form and the related POLR3GL in the other form. Previous research indicates that POLR3G and POLR3GL are differentially expressed, with POLR3G expression being largely restricted to embryonic stem cells (ESCs) and tumor cells in contrast to the ubiquitously expressed POLR3GL. To date, the functional differences between these two subunits remain largely unexplored, especially in vivo. Here, we show that POLR3G and POLR3GL containing Pol III complexes bind the same target genes and assume the same functions both in vitro and in vivo and, to a certain degree, can compensate for each other in vivo. Moreover, an observed defect in the differentiation ability of POLR3G knockout ESCs can be rescued by exogenous expression of POLR3GL. Interestingly, POLR3G knockout mice die at a very early embryonic stage, whereas POLR3GL knockout mice complete embryonic development without noticeable defects but die at about 3 weeks after birth with signs of both general growth defects and potential cerebellum-related neuronal defects. The different phenotypes of the knockout mice likely reflect differential expression levels of POLR3G and POLR3GL across developmental stages and between tissues and insufficient amounts of total Pol III in vivo.

2020-01-22 | GSE143969 | GEO

POLR3G and POLR3GL-RNA polymerase III target genes

Project description:POLR3G and POLR3GL-RNA polymerase III target genes

| PRJNA208073 | ENA

Expanding the map of Pol III transcriptome: eCLIP and ROPE-seq of POLR3A

2026-07-09 | GSE337525 | GEO

A combinatorial atlas of RNA Polymerase III subunit occupancy in human THP-1 cells

Project description:We present a genome-wide map of RNA Polymerase III subunit localization in human THP-1 monocytes and THP-1 derived macrophages after 72 hr exposure to PMA, as well as profiles of POLR3G and POLR3GL occupancy in THP-1 monocytes after 4 hr exposure to Pol III drug inhibitor ML-60218; 27 uM

2021-04-18 | GSE163422 | GEO

sRNA Seq NuRD subunit knockdown

2026-07-10 | GSE337524 | GEO

POLR3G Dependent PolyA+ and smallRNA Transcriptomes in Human Pluripotent Stem Cells

Project description:POLR3G is expressed at high levels in human pluripotent stem cells (hPSCs) and is required for maintenance of stem cell state through mechanisms, which are not known in detail. In order to explore how POLR3G regulates stem cell state, we have carried out deep sequencing analysis of polyA+ and smallRNA transcriptomes present in hPSCs and regulated in POLR3G dependent manner. Our data reveals that POLR3G regulates a specific subset of the hPSC transcriptome, including multiple transcripts types, such as protein coding genes, lincRNAs, miRNAs and snoRNAs. The primary function of POLR3G is in the maintenance rather than repression of transcription. The majority of POLR3G polyA+ transcriptome is regulated during differentiation and the key pluripotency factors bind to the promoters of at least 30 % of the POLR3G regulated transcripts. The POLR3G transcriptome is functionally linked to embryonic development and important tasks in cellular maintenance and function. #!/bin/bash

2017-05-10 | GSE94696 | GEO

Microarray analysis of Xenopus tRNA isoacceptor families, RNA Polymerase III target genes and selected mRNAs in dorsal embryonic segments of control embryos, or embryos either overexpressing the Polymerase III subunit Polr3G or its paralog Polr3gL at NF stage 25

Project description:Embryos were injected at the 1 or 2 cell stage with 2ng of Polr3G or Polr3gL mRNA. Embryos were grown alongside batch controls to NF stage 25 and dorsal embryonic segments were dissected to enrich for myogenic tissue. Through inclusion of selected developmental regulatory mRNAs, we aimed to use this data to investigate the relationship between any codon bias in highly expressed mRNAs with expression of their matching tRNA isoacceptors in myogenic lineages and how this changes in response to overexpression of these subunits.

2019-09-30 | E-MTAB-7736 | biostudies-arrayexpress

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