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Transcriptomic datat of senescence WI-38 cell

ABSTRACT: Senescence in WI-38 cell context was induce by RASv12 over expression Cellular senescence is a permanent cell cycle arrest that is triggered by cancer- initiating or promoting events in mammalian cells and is now considered a major tumour suppressor mechanism. Here, we did a transcriptomic analysis and compared WI-38 contol wich is a human fibroblaste cell line and WI-38 that overexpressed RASv12 a G protein that induce senescence. The goal of our project is to compare transciptomic profile of human growing fibroblast (WI-38 control) and senescent human fibroblast (WI-38 OERAS)

ORGANISM(S): Homo sapiens

PROVIDER: GSE33710 | GEO | 2011/11/16

SECONDARY ACCESSION(S): PRJNA148469

REPOSITORIES: GEO

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Transcriptomic datat of senescence WI-38 cell

Project description:Senescence in WI-38 cell context was induce by RASv12 over expression Cellular senescence is a permanent cell cycle arrest that is triggered by cancer- initiating or promoting events in mammalian cells and is now considered a major tumour suppressor mechanism. Here, we did a transcriptomic analysis and compared WI-38 contol wich is a human fibroblaste cell line and WI-38 that overexpressed RASv12 a G protein that induce senescence. The goal of our project is to compare transciptomic profile of human growing fibroblast (WI-38 control) and senescent human fibroblast (WI-38 OERAS) Comparaison WI-38 vs WI-38 OE RAS

2011-11-16 | E-GEOD-33710 | biostudies-arrayexpress

miRNA profile of WI-38 cells and their progressively transformed derivatives

Project description:microRNA array of 4 cell lines: WI-38 primary fibroblasts (“Control”), slow growers (early passage after immortalization, Slow), fast growers (extensive passaging after immortalization) and fast growers transformed by constitutively activated mutant H-RasV12 (“Ras”)

2016-01-29 | GSE77338 | GEO

miRNA profile of WI-38 cells and their progressively transformed derivatives

Project description:microRNA array of 4 cell lines: WI-38 primary fibroblasts (â??Controlâ??), slow growers (early passage after immortalization, Slow), fast growers (extensive passaging after immortalization) and fast growers transformed by constitutively activated mutant H-RasV12 (â??Rasâ??) WI-38 cells were grown in 37Â° in MEM supplemented with 10% non-heat-inactivated fetal bovine serum (Sigma), pen-strep, sodium pyruvate, L-glutamine solution (Beit HaEmek). RNA was extracted using Nucleospin miRNA kit (Macherey-Nagel), according to manufacturerâ??s instructions. microRNA array analysis was done in duplicates, using the miRNA Complete Labeling and Hyb Kit (Agilent, 5190-0456) according to the manufacturer's instructions. Briefly, for each sample 100ng RNA was dephosphorylated, denatured, labeled with Cyanine 3-pCp and purified using Micro Bio-Spin 6 Columns. Hybridization was done for 20h with Agilent SurePrint G3 Unrestricted miRNA 8x60K (Release 19.0) arrays. Arrays were scanned using an Agilent DNA microarray scanner, and analyzed using the AgiMicroRna package in R with the RMA algorithm. The function filterMicroRna was applied with the following parameters: control = TRUE, IsGeneDetected = TRUE, wellaboveNEG = TRUE, limIsGeneDetected = 50 and limNEG = 25.

2016-01-29 | E-GEOD-77338 | biostudies-arrayexpress

Effect of Diesel Exhaust Particles in Normal Human Embryonic Lung Cell (WI-38) Line

Project description:We performed RNA sequencing to identify genes and pathways induced by Diesel exhaust particles exposure in WI-38 cells.

2021-02-14 | GSE166729 | GEO

Small extracellular vesicles from WI-38 cells

Project description:A study comparing small extracellular vesicles (sEVs) from WI-38 and A549 cells. The proteome of the two sources of sEVs were compared to find proteins that could distinguish WI-38-derived sEVs from A549-derived sEVs. These protein markers were used as proof-of-principle biomarkers for a FRET-based sEV biomarker assay.

2022-05-22 | MSV000089517 | MassIVE

RNA-seq analysis of human WI-38 fibroblast cell line treated with a chalcone that promotes resistance to oxidative stress

Project description:We have identified through high-throughput screen a number of small molecules that promote resistance of human WI-38 cells to hydrogen peroxide. To look into potential mechanism of action for a chalcone hit, we analyzed transcriptional profile by RNA-seq for WI-38 cells treated with this molecule.

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Homo sapiens strain:WI-38 fibroblasts

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p53- repressed miRNAs are involved with E2F in a feed forward loop promoting proliferation

Project description:There is a distinctive miRNA expression profile between early (young) and replicative senescence (old). We observed a cluster of miRNAs that were repressed upon senescence in both MRC-5 and WI-38 fibroblast cells. miRNAs profiling in young and senescence human emryonic fibroblasts (MRC-5 and WI-38)

2010-05-19 | E-GEOD-12821 | biostudies-arrayexpress

Homo sapiens isolate:WI-38

Project description:Genome sequencing and assembly of WI-38 cell line

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p53- repressed miRNAs are involved with E2F in a feed forward loop promoting proliferation

2008-09-25 | GSE12821 | GEO