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Cross-species single cell transcriptomics in fly and beetle reveals the genetic core of brain neuroblast specification

ABSTRACT: This work defines the conserved core of TFs involved in insect brain NB specification by cross-species comparison of single-cell transcriptomics of NBs from, the red flour beetle, Tribolium castaneum, and the vinegar fly, Drosophila melanogaster. In Tribolium, we established a Gal4 enhancer trap screen system to generate a line that marks NBs. Using the 10x genomics technology, we sequenced 37,137 Tribolium NBs. We also sequenced 32,112 single nuclei of Drosophila NBs. Cross-species comparison followed by in situ hybridization led to the identification brain-specific TFs of insects. This brain NB-specific gene set confirms fundamentally different spatial patterning compared to the well-studied ventral nerve cord.

ORGANISM(S): Tribolium castaneum

PROVIDER: GSE337265 | GEO | 2026/07/06

REPOSITORIES: GEO

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