Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycobacterium tuberculosis Growth After Aerobic Expression of the DosR Regulon

ABSTRACT: Investigation of whole genome gene expression of M. tuberculosis strain H37Rv:deltaRv3133c::pEXNF-3133c following ectopic expression of a plasmid-borne copy of wildtype Rv3133c compared the same strain with Rv3133c uninduced. The M. tuberculosis H37Rv:deltaRv3133c was originally described in Mol Microbiol. 2003 May; 48(3): 833–843.

ORGANISM(S): Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv

PROVIDER: GSE33752 | GEO | 2012/01/31

SECONDARY ACCESSION(S): PRJNA148407

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Mycobacterium tuberculosis Growth After Aerobic Expression of the DosR Regulon

Project description:Investigation of whole genome gene expression of M. tuberculosis strain H37Rv:deltaRv3133c::pEXNF-3133c following ectopic expression of a plasmid-borne copy of wildtype Rv3133c compared the same strain with Rv3133c uninduced. The M. tuberculosis H37Rv:deltaRv3133c was originally described in Mol Microbiol. 2003 May; 48(3): 833–843. A six chip experiment in which total RNA from H37Rv:deltaRv3133c::pEXNF-3133c was collected after 24 hours of treatment with chemical expression inducer or vehicle control. Three separate cultures/biological replicates were interrogated for both induced and uninduced samples. Labeled cDNA was hybridized to NimbleGen spotted oligo tiling arrays covering both strands of the M. tuberculosis genome at ~200 bp intervals between 60-mer probes.

2012-01-31 | E-GEOD-33752 | biostudies-arrayexpress

Phosphoproteomics of Mycobacterium tuberculosis DosR, DosS, and DosT

Project description:Mass Spectrometry based Phosphoproteomics was carried out on M. tuberculosis wild type strain H37Rv and the isogenic mutant strains MtbΔdosR, MtbΔdosS, and MtbΔdosT that were cultured in aerobic as well as hypoxic conditions. During later the oxygen was gradually deprived in cultures that were kept static.

2019-08-31 | PXD015256 | JPOST Repository

Mycobacterium tuberculosis Sensor Kinase DosS Modulates the Autophagosome in a DosR-independent Manner [II]

Project description:Transcriptional profiling of bacterial RNA samples from infected Rhesus Bone Marrow Derived Macrophages (RhBMDMs) compared to RNA isolated from respective in vitro grwon cultures viz M. tuberculosis H37Rv or Isogeneic mutant MtbdosS

2018-08-22 | GSE118867 | GEO

Mycobacterium tuberculosis whiB4 mutant expression

2012-07-24 | GSE37840 | GEO

Gene expression profling of Mycobacterium tuberculosis H37Rv and H37RvΔdosSΔdosT in 0.2% glucose/acetate under aerobic/hypoxic conditions

Project description:The present study reports the gene expression data of Mycobacterium tuberculosis H37Rv and H37RvΔdosSΔdosT (DKO) grown on 0.2 % acetate/glucose under aerobic/hypoxic conditions. Acetate was reported to be present in granulomas of Mycobacterium tuberculosis infected guinea pigs which are also hypoxic. By exposing Mycobacterium tuberculosis H37Rv and H37RvΔdosSΔdosT to different combinations of granulomatous stresses (acetate/glucose and aerobic/hypoxic conditions) alongwith other experimental data, we were able to delineate a new signaling pathway that activates DevR (DosR) regulon through Acetyl phosphate. The presence of two pathways highlights the importance of targeting DevR and not DevS/DosT for intercepting DevRST signalling cascade.

2019-01-01 | GSE117428 | GEO

LIX48 (H37Ra-devR overexpression) versus LIX47 (control strain) and LIX50 (H37Rv-devR overexpression) versus LIX49 (control strain)

Project description:Transcription analysis of M. tuberculosis H37Ra devR overexpressing strain (LIX48) versus empty vector control strain (LIX47); H37Rv devR overexpressing strain t (LIX50) versus empty vector control strain (LIX49) to study the effect of DevR overexpression

2018-08-31 | GSE103551 | GEO

Genome-wide profiling of miRNA expression dynamics in monocyte-derived dendritic cells following infection with a panel of intra-cellular bacteria

Project description:We report the application of RNA sequencing to assess the expression dynamics of miRNAs and their isoforms over time upon infection with a panel of six intracellular bacteria (Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis Beijing strain GC1237, Mycobacterium bovis BCG, Salmonella typhimurium strain Keller, Staphloccocus epidermidis and Yersinia pseudotuberculosis)

2015-02-18 | GSE64142 | GEO

furB knockout

Project description:We used DNA microarrays to define the M. tuberculosis FurB regulon. For this porpouse we constructed a M. tuberculosis strain where furB was disrupted and its transcriptional profile was compared on DNA microarray with the transcriptional profile of its parental strain (H37Rv). A total of 32 genes included in 16 putative transcription units were shown to be up-regulated in the mutant relative to H37Rv. No repressed genes were identified. Seven representative genes were chosen from the microarray experiments and their expression was measured by quantitative RT-PCR using sigA as invariant internal control. In support to the gene expression profiling data, the mRNA levels of all selected genes was clearly higher in the furB mutant strain relative to H37Rv Keywords: comparative genomic hybridization

2006-09-15 | GSE5815 | GEO

Transcriptome analysis of MazF6 overexpression in Mycobacterium tuberculosis

Project description:We report the effects of MazF6 overexpression on Mycobacterium tuberculosis H37Rv strain. The total RNA was isolated from M. tuberculosis harboring either pTetR-int or pTetR-Int-mazF6 the expression was induced with 50 ng/ml anhydrotetracycline for 24 hrs.

2022-04-01 | GSE182591 | GEO

Mycobacterium tuberculosis whiB4 mutant expression

Project description:Comparison of gene expression profile of the whiB4 mutant strain of Mycobacterium tuberculosis with the wild type Mycobacterium tuberculosis H37RV Mtb WhiB4 mutant mRNA was compared with the mRNA of wtMtb H37RV under aerobic conditons Aerbic conditions OD600 nm of 0.4, MtbWhiB4KO vs wtMtb, biological replicates: 3 wt Mtb H37RV and 3 MtbWhiB4 KO

2012-07-23 | E-GEOD-37840 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data