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Over-expression of the transcription factor ARID3A (Bright)

ABSTRACT: Retroviral contructs containing the full-length murine ARID3A (aka Bright) gene and a GFP gene or the GFP gene alone were used to electroporate the human RAJI B cell line. GFP positive cells were sorted and expanded in vitro. Differences in gene expression between the constructs were identified.

ORGANISM(S): Homo sapiens

PROVIDER: GSE33777 | GEO | 2013/11/15

SECONDARY ACCESSION(S): PRJNA148597

REPOSITORIES: GEO

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Over-expression of the transcription factor ARID3A (Bright)

Project description:Retroviral contructs containing the full-length murine ARID3A (aka Bright) gene and a GFP gene or the GFP gene alone were used to electroporate the human RAJI B cell line. GFP positive cells were sorted and expanded in vitro. Differences in gene expression between the constructs were identified. Total RNA was isolated from 4 independent cell culture replicates of each retroviral construct.

2013-11-15 | E-GEOD-33777 | biostudies-arrayexpress

Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

Project description:Bright null mouse embryonic stem cells and spontaneously reprogrammed Bright null cells compared to WT mouse embryonic stem cells;Work further described in Popowski et. al 2013 Bright null mouse embryonic stem cells and wildtype mouse embryonic stem cells differentiated in embryoid bodies at day 6 and day 15

2013-11-25 | GSE52692 | GEO

Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

2013-11-25 | E-GEOD-52692 | biostudies-arrayexpress

CMK LC-MS/MS after CoIP of ARID3A

Project description:In order to determine proteins interacting with ARID3A in the ML-DS cell line CMK, we performed CoIP of endogenous ARID3A followed by LC-MS/MS

2023-03-11 | PXD025027 | Pride

10x Genomics single-cell RNA-seq of isolated Adult ID4-EGFP-bright mouse spermatogonia treated with Rapamycin

Project description:To reveal distinct transcriptome changes among ID4-EGFP-bright adult mouse spermatogonia associated with mTORC1 activity, single-cell transcriptomes were generated from GFP-bright/CD9-bright spermatogonia from adult mice in three groups: control (untreated), 2 days of Rapamycin treatment (Rapamycin) and 2 days Rapamycin plus 1 day washout (Rapamycin_Release). Based on transplantation studies performed previously, ID4-EGFPbright cells are highly enriched for SSCs. We used the 10x Genomics Chromium to perform single-cell RNA-seq.

2021-02-18 | GSE152930 | GEO

Arid3a is required for mammalian placenta development

Project description:Previous studies in the mouse indicated that Arid3a plays a critical role in the first cell fate decision required for generation of trophectoderm (TE). Here, we demonstrate that Arid3a is widely expressed during mouse and human placentation and essential for early embryonic viability. Arid3a is located within trophoblast giant cells and other trophoblast-derived cell subtypes in the junctional and labyrinth zones of the placenta. Conventional Arid3a knockout embryos suffer restricted intrauterine growth with sever defects in placental structural organization. Arid3a null placentas show aberrant expression of subtype-specific markers as well as significant alteration in inflammatory response-related genes, cytokines and chemokines. We provide evidence that BMP4-mediated induction of trophoblast stem (TS)-like cells from human induced pluripotent (iPS) stem cells results in ARID3A upregulation and cytoplasmic to nuclear translocation. Overexpression of ARID3A in human iPS and BMP4-mediated TS-like cells up-regulated TE markers, whereas pluripotent markers were down-regulated. Our results indicate that the roles of Arid3a are conserved and essential for mammalian placental development through regulation of both intrinsic and extrinsic developmental programs. Placentas of E10.5 and E11.5 wild type (WT) and Arid3a-/- mice were generated by deep sequencing, using Illumina

2016-07-01 | E-GEOD-79638 | biostudies-arrayexpress

Analysis of ARID3A regulated genes in K562 cells

Project description:To test the function and regulated genes of ARID3A gene, we conducted lentivirus-mediated short hairpin RNA (shRNA) against ARID3A in K562 cell line.

2023-06-01 | E-MTAB-12603 | biostudies-arrayexpress

Arid3a is required for mammalian placenta development

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ARID3a Gene Profiles are Strongly Associated with Human Interferon Alpha Production

Project description:Percentages of ARID3a-expressing low density neutrophils in SLE patients correlate with lupus disease activity and Type I IFN production. ARID3a protein levels also correlate with interferon expression in plasmacytoid dendritic cells. Gene profiles mechanistically link ARID3a with inflammatory pathway regulation.

2018-09-26 | GSE117836 | GEO

The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis [CUT&RUN]

Project description:The purpose of this study was to decipher the molecular function of ARID3A. We leveraged gene expression (RNA-Seq) and chromatin profiling (ATAC-Seq and CUT&RUN) to evaluate gene expression changes upon restoring Arid3a expression in the context of the Gata1s mutation and miR-125b overexpression

2022-04-20 | GSE181568 | GEO

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