Transcriptomics,Genomics

Dataset Information

26

Solanaceae comparative gene expression profiling


ABSTRACT: Six different Solanaceae species, Potato (Solanum tubersosum), Tomato (Lycopersicum esculentum), Pepper (Capsicum annuum), Tobacco (Nicotiana tabaccum), Petunia and Nicotiana benthiamana were grown at 25C, 16h light and 8h darkness. Mature leaves were harvested after 4-6 weeks. RNA was isolated using Qiagen RNeasy. Tomato, pepper, petunia, tobacco and N. benthamiana samples were hybridized against potato samples. Keywords: Solanaceae comparative gene expression profiling Overall design: Six different Solanaceae species, Potato (Solanum tubersosum), Tomato (Lycopersicum esculentum), Pepper (Capsicum annuum), Tobacco (Nicotiana tabaccum), Petunia and Nicotiana benthiamana were grown at 25C, 16h light and 8h darkness. Mature leaves were harvested after 4-6 weeks. RNA was isolated using Qiagen RNeasy. Tomato, pepper, petunia, tobacco and N. benthamiana samples were hybridized against potato samples.

INSTRUMENT(S): Potato 10k cDNA array version 2

SUBMITTER: Li Zheng  

PROVIDER: GSE3380 | GEO | 2005-10-03

SECONDARY ACCESSION(S): PRJNA93511

REPOSITORIES: GEO

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Publications

Comparative analyses of six solanaceous transcriptomes reveal a high degree of sequence conservation and species-specific transcripts.

Rensink Willem Albert WA   Lee Yuandan Y   Liu Jia J   Iobst Stacy S   Ouyang Shu S   Buell C Robin CR  

BMC genomics 20050914


BACKGROUND: The Solanaceae is a family of closely related species with diverse phenotypes that have been exploited for agronomic purposes. Previous studies involving a small number of genes suggested sequence conservation across the Solanaceae. The availability of large collections of Expressed Sequence Tags (ESTs) for the Solanaceae now provides the opportunity to assess sequence conservation and divergence on a genomic scale. RESULTS: All available ESTs and Expressed Transcripts (ETs), 449,224  ...[more]

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