3-Hydroxyanthranilic acid suppresses colorectal cancer growth and enhances 5-fluorouracil sensitivity via the YY1-p53 and YY1-DKK4 axes [RNA-Seq 3HAA_HCT116]

ABSTRACT: In spite of its reported tumor-growth-inhibition activity, the role of the tryptophan metabolite 3-hydroxyanthranilic acid (3-HAA) in colorectal cancer (CRC) remains unclear. Here, 3-HAA exhibited depleted levels in CRC tissues, and its exogenous administration effectively suppressed the proliferation of multiple CRC cell lines. Through its binding to the Yin Yang 1 (YY1) transcription factor, it disrupted the YY1–p53 interaction, preserved p53 stability, and induced cell cycle arrest. Notably, in vitro, 3-HAA reduced chemoresistance to the common CRC-chemotherapy drug 5-fluorouracil (5-FU) via the YY1–DKK4 axis. Consistently, in vivo, combined 3-HAA–5-FU treatment effectively inhibited CRC progression, highlighting the potential of 3-HAA for CRC therapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE338052 | GEO | 2026/09/11

REPOSITORIES: GEO