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Anti-Inflammatory Effects of Epidermal Growth Factor on the Immature Human Intestine

ABSTRACT: The inflammatory response of preterm infants' intestine underlines its inability to respond to hemodynamic stress, microbes and nutrients. Recent evidence suggests that exogenous epidermal growth factor (EGF) exerts a therapeutic influence on neonatal enteropathies. However, the molecular mechanisms underlying the beneficial effects of EGF remain to be clarified. The purpose of this study was to evaluate the impact of EGF on the gene expression profiles of the developing human small and large intestine at mid-gestation in serum-free organ cultures using Illumina microarrays.

ORGANISM(S): Homo sapiens

PROVIDER: GSE33806 | GEO | 2012/01/11

SECONDARY ACCESSION(S): PRJNA148125

REPOSITORIES: GEO

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Anti-Inflammatory Effects of Epidermal Growth Factor on the Immature Human Intestine

Project description:The inflammatory response of preterm infants' intestine underlines its inability to respond to hemodynamic stress, microbes and nutrients. Recent evidence suggests that exogenous epidermal growth factor (EGF) exerts a therapeutic influence on neonatal enteropathies. However, the molecular mechanisms underlying the beneficial effects of EGF remain to be clarified. The purpose of this study was to evaluate the impact of EGF on the gene expression profiles of the developing human small and large intestine at mid-gestation in serum-free organ cultures using Illumina microarrays. Differential functional effects of EGF along the human fetal intestine [Illumina] Transcriptional profiling of small and large intestinal explants cultured in the absence (control) or in the presence of EGF (50 ng/mL).

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Project description:Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC) but specific biomarkers for diagnosis and effective treatment are lacking. Neutrophil extracellular traps (NETs) are related to sepsis in adults but not investigated in infant conditions. This is the first proteome study to document that circulating NETs are involved in neonatal LOS and NEC. cfDNA and NET proteins may provide new potential diagnostic markers for these diseases.

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