Transcriptomics,Genomics

Dataset Information

36

Bi-phenotypic B-lymphoid/myeloid cells expressing low levels of Pax5 - potential targets of BAL development


ABSTRACT: Retroviral transduction of Pax5-deficient pro/preB cell lines with a doxycycline-inducible (TetON) form of the human Pax5 (huPax5) gene yielded cell clones which could be induced to different levels of huPax5 expression. Clones inducible to high levels developed B220+/CD19/+IgM+ B cells, while clones with low levels differentiated to B220+/CD19- /CD11b+/Gr-1- B-lymphoid/myeloid “bi-phenotypic” cells in vitro and in vivo. “Bi-phenotypic” cells could also be developed from high level-inducible cells by lower concentrations of doxycycline, inducing lower levels of huPax5 in vitro. Microarray analyses of genes expressed at these lower levels of huPax5 identified C/ebpα, C/ebpδ, Pu.1, Csf1r, Csf2r and Gata-3 as myeloid-related genes selectively expressed in the pro/preB cells which can develop under myeloid/lymphoid conditions to “bi-phenotypic” cells. Therefore, reduced expression of huPax5 during the induction of early lymphoid progenitors to B-lineage-committed cells can fix this cellular development at a stage that has previously been seen during embryonic development and in ALL-like “bi-phenotypic” acute leukemias (BAL). Overall design: Microarray experiments were performed as dual-color hybridizations. In order to compensate specific effects of the dyes and to ensure statistically relevant data analysis, a color-swap dye-reversal was performed.

INSTRUMENT(S): Agilent-012694 Whole Mouse Genome G4122A (Feature Number version)

SUBMITTER: Hans-Joachim Mollenkopf  

PROVIDER: GSE33875 | GEO | 2012-08-14

SECONDARY ACCESSION(S): PRJNA148343

REPOSITORIES: GEO

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Publications

Biphenotypic B-lymphoid/myeloid cells expressing low levels of Pax5: potential targets of BAL development.

Simmons Szandor S   Knoll Marko M   Drewell Christopher C   Wolf Ingrid I   Mollenkopf Hans-Joachim HJ   Bouquet Corinne C   Melchers Fritz F  

Blood 20120827 18


The expression of Pax5 commits common lymphoid progenitor cells to B-lymphoid lineage differentiation. Little is known of possible variations in the levels of Pax5 expression and their influences on hematopoietic development. We have developed a retroviral transduction system that allows for the study of possible intermediate stages of this commitment by controlling the levels of Pax5 expressed in Pax5-deficient progenitors in vitro and in vivo. Retroviral transduction of Pax5-deficient pro-/pre  ...[more]

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