RNA profiles of hematopoietic stem cells before and after long-term culture [RNA-Seq]

ABSTRACT: Self-renewing hematopoietic stem cells (HSCs) during hematopoietic regeneration showed lower intracellular glutamate (Glu) levels compared with dividing progenitor cells in the steady state and HSCs attenuate stem cell function during ex vivo culture. R162, an inhibitor of glutamine dehydrogenase (Glud1) that catabolize Glu into α-ketoglutarate, contributed to stem cell maintenance during ex vivo culture. These data suggest that lower intracellular Glu levels during HSC division contributed to self-renewing divisions by suppressing Glud1-mediated reactions.

ORGANISM(S): Mus musculus

PROVIDER: GSE338821 | GEO | 2026/07/17

REPOSITORIES: GEO