RBMS1 Suppresses Ovarian Cancer Malignant Progression Through Post-transcriptional regulation expression of NEDD4

ABSTRACT: Ovarian cancer (OC) is the deadliest gynecologic malignancy of the female reproductive system, with the molecular mechanisms driving its malignant progression not fully understood. This study aimed to identify tumor-suppressive RNA-binding proteins (RBPs) and characterize their post-transcriptional regulatory roles in OC. We screened for progression-associated RBPs through integrative analysis of multi-omics datasets from TCGA, GTEx, CPTAC, and TNMplot, and validated RBMS1 expression in clinical OC tissues via immunohistochemistry. The functional effects of RBMS1 on OC cell proliferation, migration, and invasion were assessed via in vitro and in vivo gain- and loss-of-function assays. Downstream targets of RBMS1 were identified by LC-MS/MS and RIP-seq, with regulatory relationships confirmed by rescue experiments. The deposited RIP-seq data provide genome-wide RBMS1-bound RNA profiles in OC cells. Our results show that RBMS1 is downregulated in OC and exerts tumor-suppressive effects through a novel RBMS1–NEDD4 regulatory axis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE338829 | GEO | 2026/07/21

REPOSITORIES: GEO