WT and exa1 mutant whole-genome transcriptome analysis under normal, salt and heat stress
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE339141 | GEO | 2026/07/22
REPOSITORIES: GEO
Similar Datasets
Project description:To elucidate the role of EXA1 in response to abiotic stress, we conducted whole-genome transcriptome analysis using 9-day-old seedlings of both wild-type (WT) and exa1-1 mutant lines under normal, salt, and heat stress conditions. Our findings indicate that EXA1 functions as a key regulator of gene expression associated with both stress adaptation and plant growth.
2026-07-22 | GSE339580 | GEO
Project description:Purpose: To elucidate the role of EXA1 in the response to abiotic stress, we examined the possible effects of salt and heat stresses on Arabidopsis translatome using polysome profiling. Conclusion: We found that the translatome is partly and rapidly reprogrammed in response to salt and heat stresses, and such translatome reprogramming is EXA1-dependent.
2026-07-22 | GSE339320 | GEO
Project description:High salinity is one of the major environmental factors, which hampers plant growth, development and productivity. To better understand the regulatory mechanisms by which plants cope with salt stress, we used genetic approaches to identify salt hypersensitive mutant 9 (sahy9), a new allele of apum23, in Arabidopsis thaliana. The sahy9/apum23 mutant seedlings display postgemination developmental arrest and later become bleached under agar plates supplemented with various salt stressors. Transcriptomic and proteomic analyses of the salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes with similar functional categories, primarily including cellular and metabolic processes, and abiotic and biotic stress responses. However, the consistency of gene expression at both transcript and protein levels is low (), suggesting the involvement of posttranscriptional processing in salt response. Furthermore, the altered gene/protein expression mediated by SAHY9/APUM23 in salt sensitivity is involved in several functional groups, particularly in ABA biosynthesis and signaling, abiotic stress response, LEA proteins, and ribosome biogenesis-related genes. Importantly, NCED3, a key gene involved in ABA biosynthesis, and major ABA responsive marker genes, such as RD20 and RD29B, are down-regulated at both transcript and protein levels in sahy9/apum23 under salt stress. Consistently, lower contents of ABA and proline, and expression changes of a subset of LEA proteins also support the nature of sahy9/apum23 showing salt hypersensitivity. Collectively, these data suggest that SAHY9/APUM23-mediated salt response is associated with ABA signaling pathway and its downstream stress responsive or tolerant genes.
2018-02-20 | GSE99664 | GEO
Project description:Seed germination is an important process that begins new life in plant. To ensure that seedlings grow under optimal condition after germination, seed germination should be precisely regulated by endogenous and environmental factors. Salinity is the major abiotic stress that often adversely impacts plants by inhibiting seed germination, growth and development. Comparative transcriptome analyses of salt-treated seedlings and seeds revealed that although some of the well-known salt-stress marker genes, including RD29a and DREB2A were not induced under salt, genes that can be categorized in similar GO terms involving with stress responsiveness are changed both in seeds and seedlings in response to salt treatment.
2025-11-30 | GSE282389 | GEO
Project description:Abiotic stress is a major factor for crop productivity, a problem likely to be exacerbated by climate change. Improving the tolerance to environmental stress is one of the most important goals of crop breeding programmes. While the early responses to abiotic stress in plants are well studied, plant adaptation to enduring or recurring stress conditions has received little attention. This project investigates the molecular mechanism of the maintenance of acquired thermotolerance as a model case of stress memory in Arabidopsis. Arabidopsis seedlings acquire thermotolerance through a heat treatment at sublethal temperatures. To investigate the underlying mechanisms, we are investigating changes in the transcriptome at two timepoints after a heat acclimation treatment using Arabidopsis thaliana seedlings. Microarrays were used to compare gene expression at two timepoints after a heat acclimation treatment.
2017-06-01 | GSE83136 | GEO
Project description:The effect of the abiotic stresses, heat, cold and salt on gene expression in Arabidopsis thaliana
2006-03-21 | E-TIGR-2 | biostudies-arrayexpress
Project description:Potato seedlings were subjected to cold, heat and salt stress. Expression profiles were captured at three different time-points, 3h, 9h and 27h from two different tissues, roots and leaves. The experiment was preformed independently three times. Commercially available true potato seeds (Variety Gilroy) were germinated on rafts floating on hydroponic medium in Magenta boxes. Plants were grown for 5 weeks prior to stress application under long day conditions (16h light and 8h dark) at 25C with gentle agitation. To initiate stress the medium was replaced with fresh medium pre-chilled to 4C (cold stress), pre-heated to 35C (heat stress) or supplemented with 100mM NaCl (salt stress). Cold and heat stress were maintained for the duration of the experiment by placing the Magenta boxes on ice or in a water-bath at 35C. For every individual sample two boxes of plants were used pooling a total of 6 plants per sample. For each time-point a single control sample was used by changing the media in a similar way as for the stress induction. A total of six boxes were combined for the pooled reference samples. Plants were harvested at the appropriate time and snap-frozen in liquid nitrogen. Roots and aerial tissue was separated prior to freezing. The tissue was stored at -80C freezer until isolation. Total RNA was isolated using RNeasy isolation kit. The intactness of the RNA was verified on gel and the concentration was adjusted to 3ug/ul by ethanol precipitation and re-suspension. Keywords: Direct comparison
2007-09-01 | GSE8205 | GEO
Project description:Abiotic stress is a major factor for crop productivity, a problem likely to be exacerbated by climate change. Improving the tolerance to environmental stress is one of the most important goals of crop breeding programmes. While the early responses to abiotic stress in plants are well studied, plant adaptation to enduring or recurring stress conditions has received little attention. This project investigates the molecular mechanism of the maintenance of acquired thermotolerance as a model case of stress memory in Arabidopsis. Arabidopsis seedlings acquire thermotolerance through a heat treatment at sublethal temperatures. To investigate the underlying mechanisms, we are investigating changes in the transcriptome at two timepoints after a heat acclimation treatment using Arabidopsis thaliana seedlings. Microarrays were used to compare gene expression at two timepoints after a heat acclimation treatment.
2019-04-01 | GSE112161 | GEO
Project description:MicroRNAs (miRNAs) are a class of endogenous small RNAs that play important roles in growth, development, and environmental stress response processes in plants. Ulmus pumila is a typical deciduous broadleaved tree species of north temperate, and is widely distributed in central and northern Asia, which has important economic and ecological value. With the spread and aggravate of soil salinisation, salt stress has become a major abiotic stress that highly affects the normal growth and development of U. pumila. However, to date, no investigation into the influence of salt stress on U. pumila miRNAs has been reported. To identify miRNAs and predict their target mRNA genes under salt stress, three small RNA libraries were generated and sequenced from CK (without salt stress), LSS (light salt stress for a short time) and MSL (medium-heavy salt stress for a long time) roots of U. pumila seedlings. Through integrative analysis, 245 conserved miRNAs representing 30 families and 64 novel miRNAs were identified, of which 89 exhibited altered expression level under salt stress, and 232 potential targets for the miRNAs were predicted and annotated in U. pumila. The expressions of six differentially expressed miRNAs were validated by qRT-PCR. These salt responsive miRNAs may play crucial roles in U. pumila defense against salt stress, and our miRNA data provides valuable information regarding further functional analysis of miRNAs involved in salt tolerance of U. pumila and other forest tree species.
2018-12-31 | GSE84937 | GEO
Project description:Salt stress is one of the abiotic stresses that adversely affect plant growth and agricultural productivity all over the word. Root is the organ that immediately suffers salt stress in soil, and thus the ability of roots to adapt to high salinity is critical for salt stress tolerance in plants. During a long-term evolution, plants have developed a variety of strategies to respond to salt stress. The mechanisms of salt stress response are complicated and are stilly largely unknown. In this study, through the screening of Arabidopsis mutants that are sensitive to salt stress, we identified a mutant itpk4, that displayed reduced root growth, reduced seeds germination, and increased root hairs under salt stress compared with wild type plants. in future, the molecular mechanism underlying the role of ITPK4 in root elongation under high.
2024-12-31 | GSE252395 | GEO