Transcriptomic profiling of ATP6AP1 knockdown in activated human hepatic stellate LX-2 cells

ABSTRACT: Hepatic stellate cell activation is a major driver of extracellular matrix deposition during liver fibrosis. This study investigated the transcriptional changes induced by ATP6AP1 depletion in human hepatic stellate LX-2 cells. LX-2 cells were transfected with ATP6AP1-targeting siRNA or non-targeting control siRNA, with three independent biological replicates per condition. Total RNA was extracted and subjected to RNA sequencing. Sequencing reads were aligned to the human reference genome GRCh38.p10, and differential gene-expression analysis was performed using DESeq2. The dataset was generated to identify the cellular pathways regulated by ATP6AP1 depletion, with particular emphasis on hepatic stellate cell activation, cellular senescence, cell-cycle arrest and Notch signaling

ORGANISM(S): Homo sapiens

PROVIDER: GSE339298 | GEO | 2026/07/25

REPOSITORIES: GEO