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Single-cell RNA sequencing of EGFR/RB1/TP53-mutant LUAD cell lines with and without PHOX2B overexpression to assess neuroendocrine phenotype induction

ABSTRACT: EGFR/RB1/TP53-mutant LUAD cell lines (H1975 and PC9) with and without PHOX2B overexpression were profiled to assess induction of the neuroendocrine (NE) phenotype. Cell lines transduced with lentiviral PHOX2B cDNA or empty vector (LV105) were analyzed by single-cell RNA sequencing (10x Chromium) with hashtag oligo (HTO) multiplexing (CellPlex). Conditions include parental H1975 and PC9 LUAD cell lines as well as engineered TP53/RB1 double-knockout variants (CYW). Four biological replicates were performed across 8 pooled sequencing runs (32 demultiplexed samples total).

ORGANISM(S): Homo sapiens

PROVIDER: GSE339453 | GEO | 2026/08/19

REPOSITORIES: GEO

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