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HEK293 polysome profiling of ribosome-load remodeling by minocycline

ABSTRACT: Polysome-fraction RNA sequencing of HEK293 cells treated with vehicle control or minocycline. Cytoplasmic lysates were separated into monosome, light-polysome, and heavy-polysome fractions, ERCC spike-ins were added after fractionation and before RNA extraction, and fraction RNA was sequenced to quantify transcript ribosome-load state. The processed matrix reports control fraction distributions, fraction-enrichment classifications, polyEN, and mean ribosome load (MRL).

ORGANISM(S): Homo sapiens

PROVIDER: GSE339469 | GEO | 2026/07/26

REPOSITORIES: GEO

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HEK293 polysome profiling of ribosome-load remodeling by minocycline

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