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Saccharomyces cerevisiae and propolis sensitivity

ABSTRACT: The main objective was to identify genes regulated after the BY4742 yeast cells were exposed to 0.125% for 5 or 10 min. The experiment was further valited by microbiological assays.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE33971 | GEO | 2011/11/29

SECONDARY ACCESSION(S): PRJNA150527

REPOSITORIES: GEO

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Saccharomyces cerevisiae and propolis sensitivity

Project description:The main objective was to identify genes regulated after the BY4742 yeast cells were exposed to 0.125% for 5 or 10 min. The experiment was further valited by microbiological assays. The control for this experiment that used 0.125% propolis as a treatment have 0.68% ethanol for 5 or 10 min (what corresponds to 60% alcoholic extract for 0.125% propolis). For analysis, the cells were exposed to 0.125% for 5 or 10 min were compared to the control.

2011-11-29 | E-GEOD-33971 | biostudies-arrayexpress

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Project description:Cadmium sulphide quantum dots (CdS QDs) are widely used in novel equipment. The relevance of the research lies in the need to develop risk assessments for nanomaterials (ENMs), using baker's yeast as model system. A whole-genome microarray experiment, performed on Saccharomyces cerevisiae (BY4742), showed how genes were regulated in response to CdS QDs.

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S. cerevisiae proteome response to heat stress - 240 min time course. C4PR_LIV

Project description:In this project, we follow the temporal proteome dynamics during heat stress response in a model organism Saccharomyces cerevisiae. Using label-free quantification we determine changes in the yeast proteome at different times after mild temperature shift, from 30˚C to 37˚C. The time points measured were 0 min (30˚C, no stress) and then at 10 min, 30 min, 60 min, 120 min and 240 min after cells were transferred to 37 °C. For each time point, four biological replicates were collected for the haploid wild type (BY4742, Mat ALPHA, his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; YJL088w::kanMX4) strain and a haploid strain with a chaperone Ssb1p deletion (ssb1Δ).

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