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Comparative expression profile of Porphyromonas gingivalis parent strain W83 and the HU protein (PG0121) mutant during exponential growth in rich medium.

ABSTRACT: HU protein regulates expression of genes involved in cell-division, iron acquisition, and transcriptional regulation in Porphyromonas gingivalis

ORGANISM(S): Porphyromonas gingivalis

PROVIDER: GSE34009 | GEO | 2011/11/30

SECONDARY ACCESSION(S): PRJNA150591

REPOSITORIES: GEO

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Comparative expression profile of Porphyromonas gingivalis parent strain W83 and the HU protein (PG0121) mutant during exponential growth in rich medium.

Project description:HU protein regulates expression of genes involved in cell-division, iron acquisition, and transcriptional regulation in Porphyromonas gingivalis Analysis involved RNA extracted from the parent strain W83 and the PG0121 (HU protein) mutant during exponential growth in rich medium. Each of the four experiments used a different preparation of RNA. Comparative expression profiles were determined by hybridizing the microarray slide with a mixture containing equal amount of cDNA generated from each strain. Dye swap experiments were performed.

2011-11-30 | E-GEOD-34009 | biostudies-arrayexpress

Expression profiling analyses of ECF sigma factor PG0162-PG1660 mutant of Porphyromonas gingivalis W83 [RNA-Seq]

Project description:The role of ECF sigma factors PG0162, PG01660 were involved in virulence regulationin Porphyromonas gingivalis was published Yuetan Dou, Devon Osbourne, Rachelle McKenzie, Hansel M Fletcher. (2010) Involvement of extracytoplasmic function sigma factors in virulence regulation in Porphyromonas gingivalis W83. FEMS Microbiology Letter, 312(1):24-32.

2017-09-14 | GSE103739 | GEO

Effect of deletion of trkA on gene expression of Porphyromonas gingivalis W83

Project description:To investigate the comprehensive function of trkA in Porphyromonas gingivalis W83, we established isogenic trkA deletion strain via homologous recombination and compared the transcriptional alteration between mutant and wild type group through RNA sequencing.

2022-11-07 | GSE210663 | GEO

Role of PG1626 in Porphyromonas gingivalis ATCC33277

Project description:Wild type Porphyromonas gingivalis strain ATCC33277 (V3176) and PG1626 - deficient mutant (V3177) were grown in iron replete conditions was used to compare to Porphyromonas gingivalis strains grown in iron chelated conditions.

2023-06-08 | GSE174493 | GEO

Comparison of Porphyromonas gingivalis W83 gene expression when grown with or without galactose

Project description:Porphyromonas gingivalis (P. gingivalis) W83 cultures in early exponential phase were supplemented with 0.1% galactose or vehicle control. The cultures were then permitted to grow anaerobically at 37°C for 3 hours, which corresponds to approximately one doubling of P. gingivalis. At this point, RNA extraction was performed using the cultures, and these samples were processed and submitted for RNA sequencing using an Illumina platform. Modest changes in gene expression were observed between cultures grown with or without galactose, and the majority of changes were associated with processes occurring at the cell membrane.

2018-12-21 | GSE124206 | GEO

Porphyromonas gingivalis W83

Project description:Expression profiling analyses of ECF sigma factor PG1660 mutant of Porphyromonas gingivalis W83

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Project description:Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity

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Project description:Expression profiling analyses of ECF sigma factor PG0162-PG1660 mutant of Porphyromonas gingivalis W83 [RNA-Seq]

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Comparison of Porphyromonas gingivalis 381 and W83 gene expression between surface translocation and biofilm mode

Project description:Porphyromonas gingivalis (P. gingivalis) 381 and W83 growing in motility condition (i.e. stabbed in soft agar culture) and on surface of solid agar culture (Biofilm) were prepared. Applied medium was BAPHK supplemented with hemin and menadione . The cultures were then permitted to grow anaerobically at 37°C for 24 hours, which corresponds to initial stages of surface translocation by P. gingivalis. At this point, RNA extraction was performed using the cultures, and these samples were processed and submitted for RNA sequencing using an Illumina platform. Significant changes in gene expression were observed in cells growing in motility and biofilm modes , and the majority of changes were associated with cell surface proteins, membrane proteins, biosynthesis of folates and bioenergetic pathways.

2019-03-31 | GSE128025 | GEO

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