Identification of target genes of ABI3 [SAP]
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE34041 | GEO | 2012/06/01
SECONDARY ACCESSION(S): PRJNA154241
REPOSITORIES: GEO
Similar Datasets
Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments (promoters) bound by ABI3 we performed array based ChIP analysis. Five ChIP-chip experiments were performed with chromatin of seeds of Arabidopsis and an anti-ABI3 antibody directed against a N-terminal fragment of ABI3, raised in rabbits. Non precipitated chromatin (input) served as control.
2012-05-31 | E-GEOD-34041 | biostudies-arrayexpress
Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments(promoters) bound by ABI3 we performed array based ChIP analysis.
2012-06-01 | GSE34033 | GEO
Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments(promoters) bound by ABI3 we performed array based ChIP analysis. Two ChIP experiments were performed with chromatin of seeds of Arabidopsis and an anti-ABI3 antibody directed against a N-terminal fragment of ABI3, raised in rabbits. Non precipitated chromatin (input) served as control.
2012-05-31 | E-GEOD-34033 | biostudies-arrayexpress
Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify genes regulated by ABI3 we performed array based transcriptome analysis of Dexamethasone inducible ABI3 transgenic seedlings. ABI3 mostly in concert with abscisic acid (ABA) was found to activate genes specifically expressed during the maturation phase of seed development.
2012-06-01 | GSE33951 | GEO
Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify genes regulated by ABI3 we performed array based transcriptome analysis of Dexamethasone inducible ABI3 transgenic seedlings. ABI3 mostly in concert with abscisic acid (ABA) was found to activate genes specifically expressed during the maturation phase of seed development. Two weeks old wildtype and 35S::ABI3::GR seedlings were treated with ABA, Dexamethasone (DEX) and ABA plus Dexamethasone for 4 h. Two biological independent experiments were performed. Wildtype was induced to control for DEX-induction of genes.
2012-05-31 | E-GEOD-33951 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE33951: Identification of target genes of ABI3 [Agilent 44k] GSE34033: Identification of target genes of ABI3 [Agilent 2x244k] GSE34041: Identification of target genes of ABI3 [SAP] Refer to individual Series
2012-05-31 | E-GEOD-34043 | biostudies-arrayexpress
Project description:Deciphering gene regulatory networks (GRNs) is a key for understanding gene expression regulations in living systems. Here, we describe the investigation of the ABSCISIC ACID INSENSITIVE 3 (ABI3) plant transcription factor GRN vicinity by a technique called Network Walking. The method involves transient transformation of protoplasts and inducible nuclear re-localization of transcription factors along with transcriptomic analysis. This genome-wide approach allowed the de novo recovery of i) direct and indirect ABI3 target genes, ii) cis-binding site preference, and iii) biological processes regulated by this canonical abscisic acid response factor. This work improves our knowledge of ABI3 action by inferring network motifs (such as Feed Forwar Loops) under its influence. The novel high-throughput-oriented technique will help accelerate GRN systems investigations in plants, as well as in other organisms.
2013-01-01 | GSE33344 | GEO
Project description:During seed maturation, the embryo accumulates nutrition storage compounds such as oil and reserve proteins, and acquires dormancy and desiccation tolerance. Arabidopsis transcription factors LEC1, LEC2, FUS3 and ABI3 are known as the master regulators of seed maturation because all these events during the seed maturation are severely affected by the respective mutants. In addition, the lec1, lec2 and fus3 mutants exhibit some heterochronic characteristics, as exemplified by the development of true leaf-like cotyledons during embryogenesis. To characterize these mutants at the whole genome expression level, microarray experiments were performed. Developing seeds were dissected from the siliques of the abi3-6 homozygous plants or the respective wild type plants (Col-0) at 12 and 16 days after flowering. Seeds samples were obtained from triplicate batches of plants and used for RNA preparation.
2014-10-01 | E-GEOD-61685 | biostudies-arrayexpress
Project description:During seed maturation, the embryo accumulates nutrition storage compounds such as oil and reservve proteins, and acquires dormancy and desiccation tolerance. Arabidopsis transcription factors LEC1, LEC2, FUS3 and ABI3 are known as the master regulators of seed maturation because all these events during the seed maturation are severely affected by the respective mutants. In addition, the lec1, lec2 and fus3 mutants exhibit some heterochronic characteristics, as exemplified by the development of true leaf-like cotyledons during embryogenesis. To characterize these mutants at the whole genome expression level, microarray experiments were performed.
2014-10-01 | GSE61685 | GEO
Project description:During seed maturation, the embryo accumulates nutrition storage compounds such as oil and reservve proteins, and acquires dormancy and desiccation tolerance. Arabidopsis transcription factors LEC1, LEC2, FUS3 and ABI3 are known as the master regulators of seed maturation because all these events during the seed maturation are severely affected by the respective mutants. In addition, the lec1, lec2 and fus3 mutants exhibit some heterochronic characteristics, as exemplified by the development of true leaf-like cotyledons during embryogenesis. To characterize these mutants at the whole genome expression level, microarray experiments were performed.
2014-10-01 | GSE61684 | GEO