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Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

ABSTRACT: Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Cells exposed to radicicol, an Hsp90 inhibitor, exhibit chromosomal instability. To assess the karyotype of cells with an acquired resistance to radicicol, array CGH was used to compare three stably resistant yeast colonies to a WT strain.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE34085 | GEO | 2011/12/31

SECONDARY ACCESSION(S): PRJNA156501

REPOSITORIES: GEO

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Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Cells exposed to radicicol, an Hsp90 inhibitor, exhibit chromosomal instability. To assess the karyotype of cells with an acquired resistance to radicicol, array CGH was used to compare three stably resistant yeast colonies to a WT strain. Single replicates of DNA prepared from radicicol-resistant yeast colonies are compared to a control WT colony.

2011-12-31 | E-GEOD-34085 | biostudies-arrayexpress

Genome structure of radicicol-induced aneuploids in yeast by array CGH

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Radicicol was shown to induce high level missegregation of an artificial chromosome (CF). This experiment examines possible aneuploidy of other (“natural”) chromosomes induced by radicicol. Red colonies (lost CF) were first screened by FACS. Possible aneuploids were then subjected to hybridization, with WT as the control.

2011-12-31 | E-GEOD-34086 | biostudies-arrayexpress

Genome structure of radicicol-induced aneuploids in yeast by array CGH

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Radicicol was shown to induce high level missegregation of an artificial chromosome (CF). This experiment examines possible aneuploidy of other (“natural”) chromosomes induced by radicicol.

2011-12-31 | GSE34086 | GEO

Hsp90 Stress Potentiates Rapid Cellular Adaptation through Induction of Aneuploidy

Project description:This SuperSeries is composed of the following subset Series: GSE34085: Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3) GSE34086: Genome structure of radicicol-induced aneuploids in yeast by array CGH Refer to individual Series

2011-12-31 | E-GEOD-34087 | biostudies-arrayexpress

Saccharomyces cerevisiae

Project description:Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

| PRJNA156501 | ENA

Transcription profiling of wild type and delta-sba1 yeast treated with radicicol to study gene regulation by sba1

Project description:We wanted to study regulation of gene regulation by sba1 (ortholog of mammalian p23) in yeast. sba1/p23 binds to Hsp90 and acts as a co-chaperone. We have a small molecule, radicicol, an analog of geldanamycin to inhibit the binding of p23 to Hsp90. Consequently, we have analyzed the gene expression profile in 4 conditions, wt, delta-sba1; and when Hsp90 function is impaired, that is, wt and delta-sba1 treated with radicicol.

2009-10-14 | E-TABM-573 | biostudies-arrayexpress

CGH analysis of mouse embryonic stem cells trisomic for chromosome 6, 8, 11,12 and 15

Project description:Aneuploidy, an uneven number of chromosomes, leads to severe developmental defects in mammals and is also a hallmark of cancer. However, whether aneuploidy is a driving cause or a consequence of tumor formation remains controversial. Paradoxically, existing studies based on aneuploid yeast and mouse fibroblasts have shown that aneuploidy is usually detrimental to cellular fitness. Here, we examined the effects of aneuploidy on mouse embryonic stem cells. Using a novel genetic scheme, we generated a series of aneuploid cell lines that each carries an extra copy of single chromosomes and then characterized the traits shared by these cell lines. All the aneuploid cell lines had rapid proliferation rates and enhanced colony formation efficiencies. They were less dependent on growth factors for self-renewal and showed a reduced capacity to differentiate in vitro. Moreover, xenografted aneuploid stem cells formed teratomas more efficiently, with features of neoplastic progression. These findings demonstrate that aneuploidy enhances the self-renewal capacities of stem cells and reduces their differentiation abilities.

2017-02-09 | GSE55500 | GEO

colonies

Project description:colonies Metagenome

| PRJNA673756 | ENA

sok2 vs wild type colonies

Project description:Time course analyses of gene expression in sok2 deleted vs wild type colonies during late developmental stages on plate. Keywords = ammonia Keywords = metabolism Keywords = yeast Keywords = stress Keywords = transcriptome Keywords: time-course

2004-07-11 | GSE1454 | GEO

Radicicol treatment of Drosophila cells

Project description:Drosophila S2 cells were treated with Heat-shock protein 90 (Hsp90) inhibitor radicicol for 15min, 30min and 1h. Poly(A) RNA was isolated and sequenced. Kinetics of transcriptional response to Hsp90 inhibition

2012-05-14 | E-GEOD-35812 | biostudies-arrayexpress

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