Balancing lipid synthesis and oxidation promotes B cell response to vaccination in Solid Organ Transplant Recipients

ABSTRACT: Pharmacologic immunosuppression is essential for preventing organ rejection and controlling autoimmunity, but profoundly impairs humoral immunity, increasing the risk of vaccine failure and infection. The mechanisms by which immunosuppressive therapies disrupt human B cell responses remain poorly defined. Here, we identified dysregulated lipid metabolism as a central determinant of impaired vaccine response in solid organ transplant recipients (SOTRs). We performed 10x genomics single cell RNA sequencing on isolated B cells from SOTRs and healthy controls before and after a third COVID-19 vaccine dose. The widely used immunosuppressive agent, mycophenolic acid (MPA) was strongly associated with vaccine non-response and induced excessive lipid synthesis, lipid accumulation, and mitochondrial stress in B cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE341347 | GEO | 2026/08/27

REPOSITORIES: GEO