Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time-series of gene expression in female murine ES cells during EpiSC differentiation

ABSTRACT: Embryonic stem cells (ESC) are derived from the inner cell mass of the blastocyst in the presence of leukemia inhibitory factor (LIF). In vivo these cells then differentiate into epi stem cells (EpiSC) that can be derived from the Epiblast in presence of Fgf2 and ActivinA. In this study, female ESCs cultured in 2i medium have been differentiated into EpiSC for 3.5 days in vitro by addition of Fgf2 and Activin A. The gene expression profile was analyzed every 4-12 h using mouse exon arrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE34243 | GEO | 2012/04/11

SECONDARY ACCESSION(S): PRJNA149761

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Time-series of gene expression in female murine ES cells during EpiSC differentiation

Project description:Embryonic stem cells (ESC) are derived from the inner cell mass of the blastocyst in the presence of leukemia inhibitory factor (LIF). In vivo these cells then differentiate into epi stem cells (EpiSC) that can be derived from the Epiblast in presence of Fgf2 and ActivinA. In this study, female ESCs cultured in 2i medium have been differentiated into EpiSC for 3.5 days in vitro by addition of Fgf2 and Activin A. The gene expression profile was analyzed every 4-12 h using mouse exon arrays. Mouse Pgk12.1 ES cells were differentiated into EpiSC, samples were taken every 4-12 hours for 84 hours in total

2012-04-10 | E-GEOD-34243 | biostudies-arrayexpress

Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

Project description:The requirements for self-renewal differ between EpiSCs and ES cells and the underlying mechanism is largely unknown. Here we show that mouse EpiSCs can be efficiently derived and robustly propagated even from single cells, using two small-molecule inhibitors: CHIR99021 and XAV939. The whole-genome microarray analyses is performed to confirm the identity of EpiSC maintained in CHIR/XAV by comparing the expression profile in EpiSC-CHIR/XAV to those in ESC maintained in 2i and EpiSC maintained in FGF2/activin. Total RNA from ESC-2i, EpiSC-CHIR/XAV, and EpiSC-FGF2/activin were extracted for microarray analisys

2011-08-18 | E-GEOD-31461 | biostudies-arrayexpress

Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

2011-08-19 | GSE31461 | GEO

RNA-seq of mouse embryonic stem cells differentiated to EpiSCs and further to presomitic mesoderm (PSM) via Wnt activation (CHIR99021) to study the influence of FGF signaling on PSM induction

Project description:Presomitic mesoderm (PSM) cells are the precursors of the somites, which flank both sides of the neural tube and give rise to the musculo-skeletal system shaping the vertebrate body. WNT and FGF signalling control the formation of both the PSM and the somites, and show a graded distribution with highest levels in the posterior PSM. We have employed reporter for the PSM control gene Tbx6 to investigate the differentiation of mouse ESCs from the naĂŻve state to PSM state. Feeder-free ESCs were seeded and grown on fibronectin (3-5 ng/ml)-coated plates in ESC medium containing LIF, PD0325901 and CHIR99021 (2i medium) to bring them to a naive state of pluripotency. Following this, the cells were brought to an EpiSC state by treating them with Activin A and FGF2. The EpiSC-like cells were then differentiated to PSM using CHIR99021 either alone or along with FGF ligands, FGF2 or FGF8 (Low concentration: 10 ng/ml or High concentration: 250ng/ml).

2016-06-01 | E-MTAB-3784 | biostudies-arrayexpress

Mus musculus

Project description:Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

| PRJNA145773 | ENA

Gene expression profiles of EpiSC-derived neural stem cell lines in comparison with EpiSC and embryonic CNS

Project description:Epiblast stem cells (EpiSCs) maintained in the medium with activin were freed from activin to elicit neural development, exposed to various strengths of Wnt signals, which may provide the initial stage of anteroposterior regional specificities, and placed in the culture condition to arrest neural development but expand cells as neural stem cells (NSCs) with epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2).

2023-07-20 | GSE237042 | GEO

Whole-genome bisulfite sequencing of two distinct interconvertible DNA methylomes of mouse embryonic stem cells

Project description:This study describes the DNA methylation profiling using whole-genome bisulfite sequencing of mouse ES cells, either derived and maintained in 2i serum-free NDiff medium, or in the presence of serum and LIF, or maintained and derived in the presence of serum and LIF and subsequently adapted to 2i serum-free NDiff medium, or maintained and derived in the presence of 2i and LIF and subsequently adapted to 2i serum.

2013-07-12 | GSE41923 | GEO

Single-cell analysis of allelic gene expression in pluripotency, differentiation and X-chromosome inactivation

Project description:We sequenced the mRNAs of embryonic stem cells (ESCs) cultured in different conditions. The two lines M (male) and F (female) used in this study were derived from E4 blastocysts of the same cross between a C57BL/6J (Mus musculus domesticus) and CAST/EiJ (Mus castaneus) male. mESCs were cultured in 2i and LIF as the ground state condition or in serum and LIF as the conventional condition. Epistem cell lines were also generated from the two lines by culturing them with Activin A and FGF2. In order to study more advanced development, we differentiated the two mESC lines through embryonic body formation to postmitotic motor neurons using retinoic acid and the smoothened agonist SAG. This differentiation process also results in the derivation of several types of interneurons. We picked single cells from all different conditions and generated sequencing libraries using the Smart-seq2 and Tn5 protocol. For simplicity, we designate the different condition as ES2i, ES, Epi and Neuron from hereon. We also obtained preimplantation inner cell mass and epiblast cells from E3.5 ICM (inner cell mass) and E4.5 blastocysts of the crossbred mice (male CAST/EiJ Ã female C57BL/6J) as well as postimplantation epiblast cells from E5.5 embryos of C57BL/6J mice Examination of gene expression profile in individual male and female embryonic stem cell lines along developmental progression

2016-07-27 | E-GEOD-74155 | biostudies-arrayexpress

Murine epiSC control vs murine epiSC differentiated into dopaminergic neurons [mRNA]

Project description:Transcriptional profiling of mouse embryonic, epiblast-derived, stem cells (epiSC). epiSC differentiated towards the dopaminergic phenotype were compared to control untreated epiSC. EpiSc were derived from a Pitx3-GFP mouse allowing visualization of dopaminergic (GFP+) differentiation efficiency.

2018-02-07 | GSE110196 | GEO

Murine epiSC control vs murine epiSC differentiated into dopaminergic neurons [miRNA]

Project description:Small-RNA profiling of mouse embryonic, epiblast-derived, stem cells (epiSC). epiSC differentiated towards the dopaminergic phenotype were compared to control untreated epiSC. EpiSc were derived from a Pitx3-GFP mouse allowing visualization of dopaminergic (GFP+) differentiation efficiency.

2018-02-07 | GSE110269 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data